Oncotarget

Research Papers:

Serum microRNA expression signatures as novel noninvasive biomarkers for prediction and prognosis of muscle-invasive bladder cancer

Xiumei Jiang _, Lutao Du, Weili Duan, Rui Wang, Keqiang Yan, Lili Wang, Juan Li, Guixi Zheng, Xin Zhang, Yongmei Yang and Chuanxin Wang

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Oncotarget. 2016; 7:36733-36742. https://doi.org/10.18632/oncotarget.9166

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Abstract

Xiumei Jiang1, Lutao Du1, Weili Duan1, Rui Wang1, Keqiang Yan2, Lili Wang1, Juan Li1, Guixi Zheng1, Xin Zhang1, Yongmei Yang1, Chuanxin Wang1

1Department of Clinical Laboratory, Qilu Hospital, Shandong University, Jinan, 250012, Shandong Province, China

2Department of Urology, Qilu Hospital, Shandong University, Jinan, 250012, Shandong Province, China

Correspondence to:

Chuanxin Wang, e-mail: [email protected]

Keywords: muscle-invasive bladder cancer, microRNA, prediction, prognosis, serum

Received: September 29, 2015    Accepted: April 16, 2016    Published: May 4, 2016

ABSTRACT

Noninvasive biomarkers for predicting the risk of muscle-invasive bladder cancer (MIBC) may expedite appropriate therapy and reduce morbidity and cost. Genome-wide miRNA analysis by Miseq sequencing followed by two phases of reverse transcription quantitative real-time PCR (RT-qPCR) assays were performed on serum from 207 MIBC patients, 285 nonmuscle-invasive bladder cancer (NMIBC) patients and 193 controls. A four-miRNA panel (miR-422a-3p, miR-486-3p, miR-103a-3p and miR-27a-3p) was developed for MIBC prediction with an area under the receiver operating characteristic curve (AUC) of 0.894 (95% CI, 0.846-0.931) for training set. Prospective evaluation of the miRNA panel revealed an AUC of 0.880 (95% CI, 0.834 to 0.917) in validation set, which was significantly higher than those of grade and urine cytology (both p < 0.05). Moreover, Kaplan-Meier analysis showed that MIBC patients with low miR-486-3p and miR-103a-3p levels had worse overall survival (p = 0.002 and p = 0.034, respectively). Cox analysis indicated miR-486-3p and miR-103a-3p were independently associated with overall survival of MIBC (p = 0.042 and p = 0.021, respectively). In conclusion, serum miRNA signatures might have considerable clinical values in predicting and providing prognostic information for MIBC.


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