Oncotarget

Research Papers:

Evaluating the diagnostic and prognostic value of circulating cathepsin S in gastric cancer

Wan-Li Liu, Dan Liu, Kai Cheng, Yi-Jun Liu, Shan Xing, Pei-dong Chi, Xiao-Hua Liu, Ning Xue, Yan-zhen Lai, Ling Guo and Ge Zhang _

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Oncotarget. 2016; 7:28124-28138. https://doi.org/10.18632/oncotarget.8582

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Abstract

Wan-Li Liu1,2,*, Dan Liu1,2,*, Kai Cheng3, Yi-Jun Liu1,2, Shan Xing1,2, Pei-dong Chi1,2, Xiao-Hua Liu1,2, Ning Xue1,2, Yan-zhen Lai1,2, Ling Guo4, Ge Zhang3

1State Key Laboratory of Oncology in Southern China, Sun Yat-sen University Cancer Center, Guangzhou, China

2Department of Clinical Laboratory Medicine, Sun Yat-sen University Cancer Center, Guangzhou, China

3Department of Microbial and Biochemical Pharmacy, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, China

4Department of Nasopharyngeal Carcinoma, Sun Yat-sen University of Cancer Center, Guangzhou, China

*These authors have contributed equally to this work

Correspondence to:

Ge Zhang, email: [email protected]

Keywords: Cat S, gastric cancer, diagnosis and prognostic biomarker, ELISA

Received: December 09, 2015     Accepted: March 28, 2016     Published: April 5, 2016

ABSTRACT

To evaluate whether serum Cathepsin S (Cat S) could serve as a biomarker for the diagnosis and prognosis of gastric cancer (GC), Enzyme-linked immuno sorbent assay (ELISA) was used to detect serum Cat S in 496 participants including healthy controls and patients with benign gastric diseases, gastric cancer, esophageal cancer, liver cancer, colorectal cancer, nasopharyngeal cancer and lung cancer. The levels of serum Cat S were significantly increased in cancer patients, especially in GC patients. The qRT-PCR, Western blotting, and immunohistochemical staining revealed the overexpression of Cat S in GC cell lines and tissues. The diagnostic value of serum Cat S for GC patients from controls resulted in an AUC of 0.803 with a sensitivity of 60.7% and a specificity of 90.0%. Moreover, the levels of serum Cat S were associated with GC tumor volume, lymphoid nodal status, metastasis status, and stages. Moreover, the patients with high levels of serum Cat S had a poorer overall survival. Univariate analysis revealed Cat S expression was a prognostic factor. The knockdown of Cat S significantly suppressed the migration and invasion of GC cells. This study suggested serum Cat S may be a potential biomarker for the diagnosis and prognosis of GC.


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