Oncotarget

Research Papers:

Read-through transcripts in normal human lung parenchyma are down-regulated in lung adenocarcinoma

Giulia Pintarelli, Alice Dassano, Chiara E. Cotroneo, Antonella Galvan, Sara Noci, Rocco Piazza, Alessandra Pirola, Roberta Spinelli, Matteo Incarbone, Alessandro Palleschi, Lorenzo Rosso, Luigi Santambrogio, Tommaso A. Dragani _ and Francesca Colombo

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Oncotarget. 2016; 7:27889-27898. https://doi.org/10.18632/oncotarget.8556

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Abstract

Giulia Pintarelli1,*, Alice Dassano1,*, Chiara E. Cotroneo1,6, Antonella Galvan7, Sara Noci1, Rocco Piazza2,3, Alessandra Pirola2, Roberta Spinelli8, Matteo Incarbone4, Alessandro Palleschi5, Lorenzo Rosso5, Luigi Santambrogio5, Tommaso A. Dragani1, Francesca Colombo1

1Department of Predictive and Prevention Medicine, Fondazione IRCCS, Istituto Nazionale dei Tumori, Milan, Italy

2Department of Health Sciences, University of Milano-Bicocca, Monza, Italy

3Hematology and Clinical Research Unit, San Gerardo Hospital, Monza, Italy

4Department of Surgery, San Giuseppe Hospital, Multimedica, Milan, Italy

5Department of Surgery, IRCCS Fondazione Cà Granda Ospedale Maggiore Policlinico, Università degli Studi di Milano, Milan, Italy

6Present Address: UCD School of Biomolecular and Biomedical Science, University College Dublin, Belfield, Dublin, Ireland

7Formerly, Department of Predictive and Prevention Medicine, Fondazione IRCCS, Istituto Nazionale dei Tumori, Milan, Italy

8Formerly, Department of Health Sciences, University of Milano-Bicocca, Monza, Italy

*These authors have contributed equally to this work

Correspondence to:

Tommaso A. Dragani, e-mail: [email protected]

Keywords: conjoined genes, gene fusion, lung adenocarcinoma, RNA-Seq, read-through transcripts

Received: November 17, 2015     Accepted: February 18, 2016     Published: April 02, 2016

ABSTRACT

Read-through transcripts result from the continuous transcription of adjacent, similarly oriented genes, with the splicing out of the intergenic region. They have been found in several neoplastic and normal tissues, but their pathophysiological significance is unclear. We used high-throughput sequencing of cDNA fragments (RNA-Seq) to identify read-through transcripts in the non-involved lung tissue of 64 surgically treated lung adenocarcinoma patients. A total of 52 distinct read-through species was identified, with 24 patients having at least one read-through event, up to a maximum of 17 such transcripts in one patient. Sanger sequencing validated 28 of these transcripts and identified an additional 15, for a total of 43 distinct read-through events involving 35 gene pairs. Expression levels of 10 validated read-through transcripts were measured by quantitative PCR in pairs of matched non-involved lung tissue and lung adenocarcinoma tissue from 45 patients. Higher expression levels were observed in normal lung tissue than in the tumor counterpart, with median relative quantification ratios between normal and tumor varying from 1.90 to 7.78; the difference was statistically significant (P < 0.001, Wilcoxon’s signed-rank test for paired samples) for eight transcripts: ELAVL1–TIMM44, FAM162B–ZUFSP, IFNAR2–IL10RB, INMT–FAM188B, KIAA1841–C2orf74, NFATC3–PLA2G15, SIRPB1–SIRPD, and SHANK3–ACR. This report documents the presence of read-through transcripts in apparently normal lung tissue, with inter-individual differences in patterns and abundance. It also shows their down-regulation in tumors, suggesting that these chimeric transcripts may function as tumor suppressors in lung tissue.


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