APE1 polymorphic variants cause persistent genomic stress and affect cancer cell proliferation
PDF | HTML | Supplementary Files | How to cite
Metrics: PDF 1322 views | HTML 2095 views | ?
Lisa Lirussi1,3, Giulia Antoniali1, Chiara D’Ambrosio2, Andrea Scaloni2, Hilde Nilsen3, Gianluca Tell1
1Laboratory of Molecular Biology and DNA Repair, Department of Medical and Biological Sciences, University of Udine, 33100 Udine, Italy
2Proteomics and Mass Spectrometry Laboratory, ISPAAM, National Research Council, 80147 Naples, Italy
3Department of Clinical Molecular Biology, University of Oslo and Akershus University Hospital, Nordbyhagen 1474, Norway
Gianluca Tell, email: [email protected]
Keywords: APE1/Ref-1, genotoxic damage, genetic variants, replication stress
Received: July 16, 2015 Accepted: March 12, 2016 Published: March 30, 2016
Apurinic/apyrimidinic endonuclease 1 (APE1) is the main mammalian AP-endonuclease responsible for the repair of endogenous DNA damage through the base excision repair (BER) pathway. Molecular epidemiological studies have identified several genetic variants associated with human diseases, but a well-defined functional connection between mutations in APE1 and disease development is lacking. In order to understand the biological consequences of APE1 genetic mutations, we examined the molecular and cellular consequences of the selective expression of four non-synonymous APE1 variants (L104R, R237C, D148E and D283G) in human cells. We found that D283G, L104R and R237C variants have reduced endonuclease activity and impaired ability to associate with XRCC1 and DNA polymerase β, which are enzymes acting downstream of APE1 in the BER pathway. Complementation experiments performed in cells, where endogenous APE1 had been silenced by shRNA, showed that the expression of these variants resulted in increased phosphorylation of histone H2Ax and augmented levels of poly(ADP-ribosyl)ated (PAR) proteins. Persistent activation of DNA damage response markers was accompanied by growth defects likely due to combined apoptotic and autophagic processes. These phenotypes were observed in the absence of exogenous stressors, suggesting that chronic replication stress elicited by the BER defect may lead to a chronic activation of the DNA damage response. Hence, our data reinforce the concept that non-synonymous APE1 variants present in the human population may act as cancer susceptibility alleles.
All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.