Oncotarget

Research Papers:

Characterization of site-specific glycosylation of secreted proteins associated with multi-drug resistance of gastric cancer

Jian Wu, Hongqiang Qin, Ting Li, Kai Cheng, Jiaqiang Dong, Miaomiao Tian, Na Chai, Hao Guo, Jinjing Li, Xin You, Mingming Dong, Mingliang Ye, Yongzhan Nie, Hanfa Zou and Daiming Fan _

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Oncotarget. 2016; 7:25315-25327. https://doi.org/10.18632/oncotarget.8287

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Abstract

Jian Wu1,*, Hongqiang Qin2,*, Ting Li1, Kai Cheng2, Jiaqiang Dong1, Miaomiao Tian1, Na Chai1, Hao Guo1, Jinjing Li1, Xin You2, Mingming Dong2, Mingliang Ye2, Yongzhan Nie1, Hanfa Zou2, Daiming Fan1

1State Key Laboratory of Cancer Biology and Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi'an 710032, China

2Key Laboratory of Separation Sciences for Analytical Chemistry, National Chromatographic R & A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China

*These authors contributed equally to this work

Correspondence to:

Daiming Fan, e-mail: [email protected]

Hanfa Zou, e-mail: [email protected]

Keywords: gastric cancer, secretome analysis, chemoresistance, glycoprotein, site-specific glycosylation

Received: October 14, 2015     Accepted: March 06, 2016     Published: March 23, 2016

ABSTRACT

Multi-drug resistance (MDR) remains a great obstacle to effective chemotherapy for gastric cancer. A number of secreted glycoproteins have been reported to be involved in the development of MDR in gastric cancer. However, whether glycosylation of secreted glycoproteins changes during MDR of gastric cancer is unclear. Our present work manifested that N-glycosites and site-specific glycoforms of secreted proteins in drug-resistant cell lines were distinctly different from those in the parental cell line for the first time. Further characterization highlighted the significance of some aberrantly glycosylated secretory proteins in MDR, suggesting that manipulating the glycosylation of specific glycoproteins could be a potential target for overcoming multi-drug resistance in gastric cancer.


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