Research Papers:
The microRNA-124-iGluR2/3 pathway regulates glucagon release from alpha cells
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Abstract
Haiyang Zhang1, Rui Liu1, Ting Deng1, Xia Wang1, Hongmei Lang2, Yanjun Qu1, Jingjing Duan1, Dingzhi Huang1, Guoguang Ying1, Yi Ba1
1Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin 300060, China
2Department of Endocrinology, Chengdu Military General Hospital, Chengdu, Sichuan 610083, China
Correspondence to:
Yi Ba, e-mail: [email protected]
Guoguang Ying, e-mail: [email protected]
Keywords: glucagon, α cell, iGluR2/3, miR-124, metabolism
Received: January 20, 2016 Accepted: March 07, 2016 Published: March 22, 2016
ABSTRACT
Glucagon, secreted from islet alpha cells, plays an important role in regulating glucose homeostasis; however, the molecular mechanism underlying this process is not fully understood. Previous studies have demonstrated that miRNAs are involved in the function of alpha cells. Glutamate promotes glucagon secretion by mediating the opening of Ca2+ channels. In this present, iGluR2 and iGluR3 levels were significantly increased in fasting-treated mouse islets. Additional studies showed that miR-124-3p simultaneously regulates the expression of iGluR2 and iGluR3 through the direct targeting of mRNA 3’UTR of these two genes. The miR-124-iGluRs pathway also contributed to the high level of glucagon secretion through long-term high glucose levels. Thus, a novel pathway comprising miRNA, glutamate and iGluRs has been demonstrated to regulate the biological process of glucagon release.
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