Oncotarget

Research Papers:

Sciellin mediates mesenchymal-to-epithelial transition in colorectal cancer hepatic metastasis

Chuan-Kai Chou, Chi-Chen Fan, Pei-Shan Lin, Pei-Yu Liao, Jia-Chen Tung, Chang-Hsun Hsieh, Mien-Chie Hung, Chung-Hsuan Chen and Wei-Chao Chang _

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Oncotarget. 2016; 7:25742-25754. https://doi.org/10.18632/oncotarget.8264

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Abstract

Chuan-Kai Chou1,*, Chi-Chen Fan2,3,*, Pei-Shan Lin4, Pei-Yu Liao1, Jia-Chen Tung5, Chang-Hsun Hsieh6, Mien-Chie Hung4,5,7, Chung-Hsuan Chen8,9,10,11, Wei-Chao Chang4,5

1National Applied Research Laboratories, National Laboratory Animal Center, Taipei, Taiwan

2Superintendent Office, Mackay Memorial Hospital, Taipei, Taiwan

3Department of Medical Laboratory Science and Biotechnology, Yuanpei University, Hsinchu, Taiwan

4Center for Molecular Medicine, China Medical University Hospital, Taichung, Taiwan

5Graduate Institute of Cancer Biology, China Medical University, Taichung, Taiwan

6Department of Orthopaedic Surgery, National Taiwan University Hospital, National Taiwan University, Taipei, Taiwan

7Department of Molecular and Cellular Oncology, The University of Texas MD, Anderson Cancer Center, Houston, TX, USA

8Genomics Research Center, Academia Sinica, Taipei, Taiwan

9Institute of Biochemistry & Molecular Biology, National Yang-Ming University, Taipei, Taiwan

10Department of Chemistry, National Taiwan University, Taipei, Taiwan

11Institute of Atomic & Molecular Sciences, Academia Sinica, Taipei, Taiwan

*These authors have contributed equally to this work

Correspondence to:

Wei-Chao Chang, e-mail: [email protected]

Keywords: colorectal cancer, hepatic metastasis, mesenchymal-to-epithelial transition, SCEL

Received: September 14, 2015     Accepted: March 11, 2016     Published: March 22, 2016

ABSTRACT

Hepatic metastasis is the major cause of mortality in colorectal cancer (CRC) patients. Using proteomic analysis, we found sciellin (SCEL) to be specifically expressed in hepatic metastatic CRC cell lines. SCEL knockdown increased CRC cell migration and invasion, while overexpression had the opposite effect. SCEL knockdown also caused cancer cells to form more invasive structures within 3D cultures, increased the mesenchymal marker vimentin, and attenuated the epithelial marker E-cadherin. SCEL increased WNT signaling by activating β-catenin and its downstream target c-myc, and activated mesenchymal-to-epithelial transition (MET) through a SCEL-β-catenin-E-cadherin axis. SCEL showed higher expression in late stage primary CRC than in its hepatic metastatic counterpart. SCEL expression is dynamically modulated by TGF-β1 and hypoxia, revealing a plastic MET mechanism for tumor colonization. Intrahepatic injection in immunodeficient mice revealed that SCEL is necessary for metastatic CRC tumor growth in the liver. These results demonstrate that SCEL is a MET inducer dynamically regulated through the metastasis process. They suggest SCEL may be a useful therapeutic target for preventing or eliminating CRC hepatic metastasis.


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