Oncotarget

Research Papers: Pathology:

Deinococcus radiodurans pprI expression enhances the radioresistance of eukaryotes

Ling Wen, Ling Yue, Yi Shi, Lili Ren, Tingting Chen, Na Li, Shuyu Zhang, Wei Yang _ and Zhanshan Yang

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Oncotarget. 2016; 7:15339-15355. https://doi.org/10.18632/oncotarget.8137

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Abstract

Ling Wen1, Ling Yue1, Yi Shi1, Lili Ren1, Tingting Chen1, Na Li1, Shuyu Zhang2, Wei Yang3 and Zhanshan Yang1

1 Department of Radiation Toxicology, School of Radiological Medicine and Protection, Medical College of Soochow University, Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou, Jiangsu, China

2 Department of Radiation Genetics, School of Radiological Medicine and Protection, Medical College of Soochow University, Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou, Jiangsu, China

3 Department of Radiobiology, School of Radiological Medicine and Protection, Medical College of Soochow University, Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou, Jiangsu, China

Correspondence to:

Wei Yang, email:

Zhanshan Yang, email:

Keywords: Deinococcus radiodurans, pprI, radioresistance, acute radiation injury, electroporation, Pathology Section

Received: August 06, 2015 Accepted: March 04, 2016 Published: March 16, 2016

Abstract

PprI accelerates radiation-induced DNA damage repair via regulating the expression of DNA repair genes and enhances antioxidative enzyme activity in Deinococcus radiodurans after radiation. The main aim of our study was to determine whether the expression of pprI gene could fulfil its DNA repair function in eukaryotes and enhance the radioresistance of eukaryotic organism or not. In this study, we constructed pEGFP-c1-pprI eukaryotic expression vector and established a human lung epithelial cell line BEAS-2B with stable integration of pprI gene. We found that pprIexpression enhanced radioresistance of BEAS-2B cells, decreased γ-H2AX foci formation and apoptosis in irradiated BEAS-2B cells and alleviated radiation induced G2/M arrest of BEAS-2B cells. Moreover, we transferred pEGFP-c1-pprI vector into muscle of BALB/c mice by in vivo electroporation and studied the protective effect of prokaryotic pprI gene in irradiated mice. We found that pprI expression alleviated acute radiation induced hematopoietic system, lung, small intestine and testis damage and increased survival rate of irradiated mice via regulating Rad51 expression in different organs. These findings suggest that prokaryotic pprI gene expression in mammalian cells could enhance radioresistance in vitro and in vivo.


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