Oncotarget

Research Papers:

MicroRNA-137 inhibits tumor growth and sensitizes chemosensitivity to paclitaxel and cisplatin in lung cancer

Hua Shen, Lin Wang, Xin Ge, Cheng-Fei Jiang, Zhu-Mei Shi, Dong-Mei Li, Wei-Tao Liu, Xiaobo Yu and Yong-Qian Shu _

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Oncotarget. 2016; 7:20728-20742. https://doi.org/10.18632/oncotarget.8011

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Abstract

Hua Shen1,6,*, Lin Wang3,4,6,*, Xin Ge3,4,6, Cheng-Fei Jiang3,4,6, Zhu-Mei Shi5,6, Dong-Mei Li3,4,6, Wei-Tao Liu3,4,6, Xiaobo Yu2, Yong-Qian Shu1,6

1Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, 210029, China

2Department of Thoracic Surgery, Shanghai General Hospital, Shanghai Jiao Tong University, Minhang, Shanghai, 200080, China

3State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, Jiangsu, 210029, China

4Department of Pathology, and Cancer Center, Nanjing Medical University, Nanjing, Jiangsu, 210029, China

5Department of Neurosurgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, 210029, China

6Collaborative Innovation Center for Cancer Medicine, Jiangsu Key Laboratory of Cancer Biomarkers, Prevention and Treatment, Nanjing Medical University, Nanjing, Jiangsu, 210029, China

*These authors contributed equally to this work

Correspondence to:

Yong-Qian Shu, e-mail: [email protected]

Xiaobo Yu, e-mail: [email protected]

Keywords: miR-137, NUCKS1, paclitaxel, cisplatin, chemosensitivity

Received: December 25, 2015     Accepted: February 14, 2016     Published: March 09, 2016

ABSTRACT

Chemotherapy resistance frequently drives tumour progression. However, the underlying molecular mechanisms are poorly characterized. In this study, we explored miR-137’s role in the chemosensitivity of lung cancer. We found that the expression level of miR-137 is down-regulated in the human lung cancer tissues and the resistant cells strains: A549/paclitaxel(A549/PTX) and A549/cisplatin (A549/CDDP) when compared with lung cancer A549 cells. Moreover, we found that overe-expression of miR-137 inhibited cell proliferation, migration, cell survival and arrest the cell cycle in G1 phase in A549/PTX and A549/CDDP. Furthermore, Repression of miR-137 significantly promoted cell growth, migration, cell survival and cell cycle G1/S transition in A549 cells. We further demonstrated that the tumor suppressive role of miR-137 was mediated by negatively regulating Nuclear casein kinase and cyclin-dependent kinase substrate1(NUCKS1) protein expression. Importantly, miR-137 inhibits A549/PTX, A549/CDDP growth and angiogenesis in vivo. Our study is the first to identify the tumor suppressive role of over-expressed miR-137 in chemosensitivity. Identification of a novel miRNA-mediated pathway that regulates chemosensitivity in lung cancer will facilitate the development of novel therapeutic strategies in the future.


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