Research Papers:

This article has been retracted. Retraction in: Oncotarget. 2016; 7(30):48851.

MMP-13 is involved in oral cancer cell metastasis

Shun-Hong Huang, Ching-Hsuan Law, Ping-Hsueh Kuo, Ren-Yu Hu, Ching-Chieh Yang, Ting-Wen Chung, Ji-Min Li, Li-Hsun Lin, Yi-Chung Liu, En-Chi Liao, Yi-Ting Tsai, Yu-Shan Wei, Chi-Chen Lin, Chien-Wen Chang, Hsiu-Chuan Chou, Wen-Ching Wang, Margaret Dah-Tsyr Chang, Lu-Hai Wang, Hsing-Jien Kung, Hong-Lin Chan and Ping-Chiang Lyu _

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Oncotarget. 2016; 7:17144-17161. https://doi.org/10.18632/oncotarget.7942

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Shun-Hong Huang1, Ching-Hsuan Law1, Ping-Hsueh Kuo2, Ren-Yu Hu1, Ching-Chieh Yang3,4, Ting-Wen Chung1, Ji-Min Li1, Li-Hsun Lin1, Yi-Chung Liu1,12, En-Chi Liao1, Yi-Ting Tsai1, Yu-Shan Wei1, Chi-Chen Lin5,6,7,8, Chien-Wen Chang9, Hsiu-Chuan Chou10, Wen-Ching Wang2, Margaret Dah-Tsyr Chang2, Lu-Hai Wang11, Hsing-Jien Kung11, Hong-Lin Chan1,2, Ping-Chiang Lyu1,2

1Department of Medical Sciences and Institute of Bioinformatics and Structural Biology, National Tsing Hua University, Hsinchu, Taiwan

2Department of Medical Sciences and Institute of Molecular and Cellular Biology, National Tsing Hua University, Hsinchu, Taiwan

3Department of Radiation Oncology, Chi-Mei Medical Center, Tainan, Taiwan

4Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan

5Institute of Biomedical Science, National Chung Hsing University, Taichung, Taiwan

6Institute of Biomedical Science and Rong Hsing Research Center for Translational Medicine, National Chung Hsing University, Taichung, Taiwan

7Department of Medical Research and Education, Taichung Veterans General Hospital, Taichung, Taiwan

8Division of Chest Medicine. Department of Internal Medicine, Changhua Christian Hospital, Changhua, Taiwan

9Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Taiwan

10Department of Applied Science, National Hsinchu University of Education, Hsinchu, Taiwan

11Institute of Molecular and Genomic Medicine, National Health Research Institutes, Miaoli County, Taiwan

12Institute of Population Sciences, National Health Research Institutes, Miaoli County, Taiwan

Correspondence to:

Hong-Lin Chan, e-mail: hlchan@life.nthu.edu.tw

Ping-Chiang Lyu, e-mail: pclyu@mx.nthu.edu.tw

Keywords: ECM, EMT, IF, MMP-13, OSCC

Received: November 23, 2015     Accepted: February 09, 2016     Published: March 06, 2016


The oral cancer cell line OC3-I5 with a highly invasive ability was selected and derived from an established OSCC line OC3. In this study, we demonstrated that matrix metalloproteinases protein MMP-13 was up-regulated in OC3-I5 than in OC3 cells. We also observed that expression of epithelial–mesenchymal transition (EMT) markers including Twist, p-Src, Snail1, SIP1, JAM-A, and vinculin were increased in OC3-I5 compared to OC3 cells, whereas E-cadherin expression was decreased in the OC3-I5 cells. Using siMMP-13 knockdown techniques, we showed that siMMP-13 not only reduced the invasion and migration, but also the adhesion abilities of oral cancer cells. In support of the role of MMP-13 in metastasis, we used MMP-13 expressing plasmid-transfected 293T cells to enhance MMP-13 expression in the OC3 cells, transplanting the MMP-13 over expressing OC3 cells into nude mice led to enhanced lung metastasis. In summary, our findings show that MMP-13 promotes invasion and metastasis in oral cancer cells, suggesting altered expression of MMP-13 may be utilized to impede the process of metastasis.

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