Oncotarget

Research Papers:

The angiogenesis in decellularized scaffold-mediated the renal regeneration

Jin Mei _, Yaling Yu, Miaozhong Li, Shanshan Xi, Sixiao Zhang, Xiaolin Liu, Junqun Jiang, Zhibin Wang, Jianse Zhang, Yuqiang Ding, Xinfa Lou and Maolin Tang

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Oncotarget. 2016; 7:27085-27093. https://doi.org/10.18632/oncotarget.7785

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Abstract

Jin Mei1,2,3,*, Yaling Yu1,2,*, Miaozhong Li4, Shanshan Xi1,2, Sixiao Zhang4, Xiaolin Liu1,2, Junqun Jiang1,2, Zhibin Wang2, Jianse Zhang1,2, Yuqiang Ding3, Xinfa Lou2, Maolin Tang1,2

1Anatomy Department, Wenzhou Medical University, Wenzhou, 325035, China

2Institute of Bioscaffold Transplantation and Immunology, Wenzhou Medical University, Wenzhou, 325035, China

3Institute of Neuroscience, Wenzhou Medical University, Wenzhou, 325035, China

4Medical School of Ningbo University, Ningbo, 315211, China

*These authors contributed equally to this work

Correspondence to:

Jin Mei, email: [email protected]

Maolin Tang, email: [email protected]

Keywords: kidney regeneration, endothelial cells, endothelial progenitor cells, decellularized scaffolds, angiogenesis

Received: November 25, 2015     Accepted: January 29, 2016     Published: February 27, 2016

ABSTRACT

There are increasing numbers of patients underwent partial nephrectomy, and recovery of disturbed renal function is imperative post partial nephrectomy. We previously have demonstrated the decellularized (DC) scaffolds could mediate the residual kidney regeneration and thus improve disturbed renal function after partial nephrectomy. However, the cellular changes including the angiogenesis in the implanted DC scaffold has not yet been elaborated. In this study, we observed that the scaffold promoted the proliferation of human umbilical vein endothelial cells (HUVEC) that adhered to the DC scaffold in vitro. We next examined the pathological changes of the implanted DC graft in vivo, and found a decreased volume of the scaffold and a dramatic angiogenesis within the scaffold. The average microvessel density (aMVD) increased at the early stage, while decreased at the later stage post transplantation. Expression level of vascular endothelial growth factor (VEGF) showed similar dynamic changes. In addition, many endothelial cells (ECs) and endothelial progenitor cells (EPCs) were distributed in the region which contained active angiogenesis in the scaffold. However, the implanted graft became fibrosis and the angiogenesis degraded at final stage roughly 8 weeks post transplantation. Our data indicate that DC scaffold can be vascularized in vivo and possible mechanisms are discussed.


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