Oncotarget

Research Papers:

AEG-1/MTDH-activated autophagy enhances human malignant glioma susceptibility to TGF-β1-triggered epithelial-mesenchymal transition

Meijuan Zou _, Wei Zhu, Li Wang, Lei Shi, Rui Gao, Yingwei Ou, Xuguan Chen, Zhongchang Wang, Aiqin Jiang, Kunmei Liu, Ming Xiao, Ping Ni, Dandan Wu, Wenping He, Geng Sun, Ping Li, Sulan Zhai, Xuerong Wang and Gang Hu

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Oncotarget. 2016; 7:13122-13138. https://doi.org/10.18632/oncotarget.7536

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Abstract

Meijuan Zou1, Wei Zhu2, Li Wang3, Lei Shi3, Rui Gao1, Yingwei Ou1, Xuguan Chen1, Zhongchang Wang4, Aiqin Jiang5, Kunmei Liu6, Ming Xiao7, Ping Ni1, Dandan Wu1, Wenping He1, Geng Sun1, Ping Li1, Sulan Zhai1, Xuerong Wang1, Gang Hu1

1Department of Pharmacology, School of Basic Medical Sciences, Nanjing Medical University, Nanjing 210029, China

2Department of Oncology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China

3Department of Breast Surgery, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China

4State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China

5Medical School of Nanjing University, Nanjing 210093, China

6Ningxia Key Laboratory of Cerebrocranial Diseases, School of Laboratory Medicine, Ningxia Medical University, Yinchuan 750004, China

7Department of Anatomy, School of Basic Medical Sciences, Nanjing Medical University, Nanjing 210029, China

Correspondence to:

Meijuan Zou, e-mail: [email protected]

Keywords: transforming growth factor-β1, astrocyte elevated gene-1, protective autophagy, epithelial mesenchymal transition, malignant glioma invasion

Received: June 18, 2015     Accepted: January 27, 2016     Published: February 20, 2016

ABSTRACT

Autophagy is a tightly regulated process activated in response to metabolic stress and other microenvironmental changes. Astrocyte elevated gene 1 (AEG-1) reportedly induces protective autophagy. Our results indicate that AEG-1 also enhances the susceptibility of malignant glioma cells to TGF-β1-triggered epithelial-mesenchymal transition (EMT) through induction of autophagy. TGF-β1 induced autophagy and activated AEG-1 via Smad2/3 phosphorylation in malignant glioma cells. Also increased was oncogene cyclin D1 and EMT markers, which promoted tumor progression. Inhibition of autophagy using siRNA-BECN1 and siRNA-AEG-1 suppressed EMT. In tumor samples from patients with malignant glioma, immunohistochemical assays showed that expression levels of TGF-β1, AEG-1, and markers of autophagy and EMT, all gradually increase with glioblastoma progression. In vivo siRNA-AEG-1 administration to rats implanted with C6 glioma cells inhibited tumor growth and increased the incidence of apoptosis among tumor cells. These findings shed light on the mechanisms underlying the invasiveness and progression of malignant gliomas.


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