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Research Papers:

Oblongifolin M, an active compound isolated from a Chinese medical herb Garcinia oblongifolia, potently inhibits enterovirus 71 reproduction through downregulation of ERp57

Mengjie Wang, Qi Dong, Hua Wang, Yaqing He, Ying Chen, Hong Zhang, Rong Wu, Xinchun Chen, Boping Zhou, Jason He, Hsiang-Fu Kung, Canhua Huang, Yuquan Wei, Jian-dong Huang, Hongxi Xu and Ming-Liang He _

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Oncotarget. 2016; 7:8797-8808. https://doi.org/10.18632/oncotarget.7122

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Abstract

Mengjie Wang1,2,*, Qi Dong2,3,9,*, Hua Wang3, Yaqing He4, Ying Chen9, Hong Zhang1, Rong Wu1, Xinchun Chen5, Boping Zhou5, Jason He8, Hsiang-Fu Kung2,3, Canhua Huang6, Yuquan Wei6, Jian-dong Huang7, Hongxi Xu1, Ming-Liang He9

1School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, China

2Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen, China

3Stanley Ho Center for Emerging Infectious Diseases, The Chinese University of Hong Kong, Hong Kong, China

4Shenzhen Center for Disease Control and Prevention (Shenzhen CDC), Shenzhen, China

5Institute of Infectious Diseases, The 3rd Peoples’ Hospital of Shenzhen, Shenzhen, China

6State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, China

7School of Biomedical Sciences, The University of Hong Kong, Hong Kong, China

8College of Letter and Sciences, University of California at Berkeley, Berkeley, CA, USA

9Department of Biomedical Sciences, City University of Hong Kong, Hong Kong, China

*These authors have contributed equally to this work

Correspondence to:

Ming-Liang He, e-mail: mlhe7788@gmail.com

Hongxi Xu, e-mail: xuhongxi88@gmail.com

Keywords: enterovirus 71, Oblongifolin M, ERp57, antiviral

Received: October 09, 2015    Accepted: January 19, 2016    Published: February 01, 2016

ABSTRACT

There is no effective drug to treat EV71 infection yet. Traditional Chinese herbs are great resources for novel antiviral compounds. Here we showed that Oblongifolin M (OM), an active compound isolated from Garcinia oblongifolia, potently inhibited EV71 infection in a dose dependent manner. To identify its potential effectors in the host cells, we successfully identified 18 proteins from 52 differentially expressed spots by comparative proteomics studies. Further studies showed that knockdown of ERp57 inhibited viral replication through downregulating viral IRES (internal ribosome entry site) activities, whereas ectopic expression of ERp57 increased IRES activity and partly rescued the inhibitory effects of OM on viral replication. We demonstrated that OM is an effective antiviral agent; and that ERp57 is one of its cellular effectors against EV71 infection.


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