Oncotarget

Research Papers:

The prognostic effects of tumor infiltrating regulatory T cells and myeloid derived suppressor cells assessed by multicolor flow cytometry in gastric cancer patients

Han Sol Choi, Sang Yun Ha, Hye-Mi Kim, Soo Min Ahn, Myung-Soo Kang, Kyoung-Mee Kim, Min Gew Choi, Joon Ho Lee, Tae Sung Sohn, Jae Moon Bae, Sung Kim and Eun-Suk Kang _

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Oncotarget. 2016; 7:7940-7951. https://doi.org/10.18632/oncotarget.6958

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Abstract

Han Sol Choi1,2,*, Sang Yun Ha3,*, Hye-Mi Kim1,2, Soo Min Ahn3, Myung-Soo Kang4,5 Kyoung-Mee Kim3, Min Gew Choi6, Joon Ho Lee6, Tae Sung Sohn6, Jae Moon Bae6, Sung Kim6, Eun-Suk Kang2

1Samsung Biomedical Research Institute, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea

2Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea

3Department of Pathology and Translational Genomics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea

4Samsung Advanced Institute for Health Sciences and Technology (SAIHST), Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea

5Samsung Biomedical Research Institute (SBRI), Center for Future Sciences, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea

6Department of Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea

*These author contributed equally to this work

Correspondence to:

Eun-Suk Kang, e-mail: eskang@skku.edu

Kyoung-Mee Kim, e-mail: kkmkys@skku.edu

Keywords: regulatory T cells, myeloid derived suppressor cells, prognosis, gastric cancer

Received: April 17, 2015     Accepted: December 26, 2015     Published: January 20, 2016

ABSTRACT

The prognostic effects of tumor infiltrating lymphocytes (TILs), especially regulatory T cells (Tregs) and myeloid derived suppressing cells (MDSCs) are inconclusive in gastric cancers. We investigated the frequencies of TILs including CD8+ T cells, CD45+CD4+CD25± FOXP3+ Tregs, CD45+CD11b+ CD14+ HLA−DR− MDSCs in 28 gastric cancer tissues by using multicolor flow cytometry. In gastric cancer tissue, the percentage of Tregs among the CD4+ T cell subset was substantially increased compared to that of Tregs among peripheral blood CD4+ T cells from the controls. High frequency of CD8+ T cells among CD3+ T cells correlated with increased overall survival (OS) (p = 0.005). High frequency of Tregs among CD4+ T cells correlated with increased OS (p < 0.001), and disease-free survival (DFS) (p = 0.039) and was an independent prognostic factor in OS (Hazard ratio: 0.047; 95% confidence interval, 0.006–0.372; p = 0.004). High frequency of MDSCs among total examined cells correlated with decreased OS (p = 0.027) and was an independent prognostic factor in OS (Hazard ratio 8.601; 95% confidence interval, 1.240−59.678; p = 0.029). We have demonstrated that high levels of Tregs among tumor-infiltrating CD4+ T cells were favorable, but an increased proportion of MDSCs was an adverse independent prognostic factor in gastric cancer. Our results may provide important insights for future immunotherapy in gastric cancer.


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