Interplay between PCBP2 and miRNA modulates ARHGDIA expression and function in glioma migration and invasion
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Xihua Lin1, Bin Yang1, Wei Liu1, Xiaochao Tan3, Fan Wu1, Peishan Hu1, Tao Jiang2, Zhaoshi Bao2, Jiangang Yuan1, Boqin Qiang1, Xiaozhong Peng1 and Wei Han1
1 Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China
2 Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
3 Department of Thoracic/Head and Neck Medical Oncology, University of Texas MD Anderson Cancer Center, Houston, Texas, USA
Wei Han, email:
Xiaozhong Peng, email:
Keywords: PCBP2, ARHGDIA, miRNA, glioma, migration and invasion
Received: September 01, 2015 Accepted: January 03, 2016 Published: January 09, 2016
RNA-RNA and protein-RNA interactions are essential for post-transcriptional regulationin normal development and may be deregulated in cancer initiation and progression. The RNA-binding protein PCBP2, an oncogenic protein in human malignant gliomas, is an essential regulator of mRNA and miRNA biogenesis, stability and activity. Here, we identified Rho GDP dissociation inhibitor α (ARHGDIA) as a target mRNA that binds to PCBP2, and we uncovered the role of ARHGDIA as a putative metastasis suppressor through analyses of in vitro and in vivo models of EMT and metastasis. Furthermore, we demonstrated that ARHGDIA is a potential target of miR-151-5p and miR-16 in gliomas. The interaction between PCBP2 and the 3’UTR of the ARHGDIA mRNA may induce a local change in RNA structure that favors subsequent binding of miR-151-5p and miR-16, thus leading to the suppression of ARHGDIA expression. PCBP2 may facilitate miR-151-5p and miR-16 promotion of glioma cell migration and invasion through mitigating the function of ARHGDIA.
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