Oncotarget

Research Papers:

Interplay between PCBP2 and miRNA modulates ARHGDIA expression and function in glioma migration and invasion

Xihua Lin, Bin Yang, Wei Liu, Xiaochao Tan, Fan Wu, Peishan Hu, Tao Jiang, Zhaoshi Bao, Jiangang Yuan, Boqin Qiang, Xiaozhong Peng and Wei Han _

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Oncotarget. 2016; 7:19483-19498. https://doi.org/10.18632/oncotarget.6869

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Abstract

Xihua Lin1, Bin Yang1, Wei Liu1, Xiaochao Tan3, Fan Wu1, Peishan Hu1, Tao Jiang2, Zhaoshi Bao2, Jiangang Yuan1, Boqin Qiang1, Xiaozhong Peng1 and Wei Han1

1 Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China

2 Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China

3 Department of Thoracic/Head and Neck Medical Oncology, University of Texas MD Anderson Cancer Center, Houston, Texas, USA

Correspondence to:

Wei Han, email:

Xiaozhong Peng, email:

Keywords: PCBP2, ARHGDIA, miRNA, glioma, migration and invasion

Received: September 01, 2015 Accepted: January 03, 2016 Published: January 09, 2016

Abstract

RNA-RNA and protein-RNA interactions are essential for post-transcriptional regulationin normal development and may be deregulated in cancer initiation and progression. The RNA-binding protein PCBP2, an oncogenic protein in human malignant gliomas, is an essential regulator of mRNA and miRNA biogenesis, stability and activity. Here, we identified Rho GDP dissociation inhibitor α (ARHGDIA) as a target mRNA that binds to PCBP2, and we uncovered the role of ARHGDIA as a putative metastasis suppressor through analyses of in vitro and in vivo models of EMT and metastasis. Furthermore, we demonstrated that ARHGDIA is a potential target of miR-151-5p and miR-16 in gliomas. The interaction between PCBP2 and the 3’UTR of the ARHGDIA mRNA may induce a local change in RNA structure that favors subsequent binding of miR-151-5p and miR-16, thus leading to the suppression of ARHGDIA expression. PCBP2 may facilitate miR-151-5p and miR-16 promotion of glioma cell migration and invasion through mitigating the function of ARHGDIA.


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