Oncotarget

Clinical Research Papers:

Comparison of analytic performances of Cellsearch and iFISH approach in detecting circulating tumor cells

Yuan Sheng, Ting Wang, Hengyu Li, Zhenzhen Zhang, Jianghao Chen, Chenyang He, Yongping Li, Yonggang Lv, Juliang Zhang, Cheng Xu, Zhen Wang, Chen Huang and Ling Wang _

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Oncotarget. 2017; 8:8801-8806. https://doi.org/10.18632/oncotarget.6688

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Abstract

Yuan Sheng2,*, Ting Wang1,*, Hengyu Li2,*, Zhenzhen Zhang3,*, Jianghao Chen1,*, Chenyang He1, Yongping Li1, Yonggang Lv1, Juliang Zhang1, Cheng Xu3, Zhen Wang4, Chen Huang5 and Ling Wang1

1 Department of Vascular and Endocrine Surgery, Xijing Hospital, The Fourth Military Medical University, Xi’an, Shaanxi, China

2 Department of Thyroid and Breast Surgery, Changhai Hospital, The Second Military Medical University, Shanghai, China

3 Biotecan Medical Diagnostics Co., Ltd, Zhangjiang Center for Translational Medicine, Shanghai,China

4 Department of Orthopedics, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaanxi, China

5 Department of Nephrology, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaanxi, China

* These authors have contributed equally to the work

Correspondence to:

Ling Wang, email:

Zhen Wang, email:

Chen Huang, email:

Keywords: breast cancer, circulating tumor cells (CTC), subtraction enrichment, cellsearch, aneuploidy

Received: December 11, 2014 Accepted: December 10, 2015 Published: December 19, 2015

Abstract

Circulating tumor cells (CTCs) have been widely used to predict the prognosis of breast cancer patients. The aim of the present study was to compare the performances of Cellsearch and immunostaining-fluorescence in situ hybridization (iFISH) in detecting CTCs in breast cancer patients. Forty-five newly diagnosed breast cancer patients and 14 healthy donors were recruited and their CTCs were detected by both Cellsearch and iFISH. Correlation between clinicopathological features and CTCs was investigated. We found that the positive rate of CTC detected by iFISH was significantly higher than by Cellsearch system (91% vs 38%). The CTC count, detected either by iFISH or Cellsearch, was not significantly associated with clinical pictures of patients with breast cancer. Therefore, we concluded that, compared to conventional Cellsearch CTC detection, in situ karyotypic identification performed by iFISH had higher detection rate. Therefore, iFISH may be more clinically useful than Cellsearch system.


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