Oncotarget

Research Papers:

VE-cadherin cleavage by ovarian cancer microparticles induces β-catenin phosphorylation in endothelial cells

Hamda Al Thawadi, Nadine Abu-Kaoud, Haleema Al Farsi, Jessica Hoarau-Véchot, Shahin Rafii, Arash Rafii and Jennifer Pasquier _

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Oncotarget. 2016; 7:5289-5305. https://doi.org/10.18632/oncotarget.6677

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Abstract

Hamda Al Thawadi1,2, Nadine Abu-Kaoud2, Haleema Al Farsi1,2, Jessica Hoarau-Véchot2, Shahin Rafii3, Arash Rafii2,3, Jennifer Pasquier2,3

1Qatar Research Leadership Program, Qatar Foundation, Doha, Qatar

2Stem Cell and Microenvironment Laboratory, Weill Cornell Medical College in Qatar, Education City, Qatar Foundation, Doha, Qatar

3Department of Genetic Medicine, Weill Cornell Medical College, NY, USA

Correspondence to:

Jennifer Pasquier, e-mail: jep2026@qatar-med.cornell.edu

Keywords: microparticles, ovarian cancer, tumor microenvironment, β-catenin, angiogenesis

Received: August 19, 2015     Accepted: November 25, 2015     Published: December 19, 2015

ABSTRACT

Microparticles (MPs) are increasingly recognized as important mediators of cell-cell communication in tumour growth and metastasis by facilitating angiogenesis-related processes. While the effects of the MPs on recipient cells are usually well described in the literature, the leading process remains unclear. Here we isolated MPs from ovarian cancer cells and investigated their effect on endothelial cells. First, we demonstrated that ovarian cancer MPs trigger β-catenin activation in endothelial cells, inducing the upregulation of Wnt/β-catenin target genes and an increase of angiogenic properties. We showed that this MPs mediated activation of β-catenin in ECs was Wnt/Frizzled independent; but dependent on VE-cadherin localization disruption, αVβ3 integrin activation and MMP activity. Finally, we revealed that Rac1 and AKT were responsible for β-catenin phosphorylation and translocation to the nucleus. Overall, our results indicate that MPs released from cancer cells could play a major role in neo-angiogenesis through activation of beta catenin pathway in endothelial cells.


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