Oncotarget

Research Papers:

SATB1 and SATB2 play opposing roles in c-Myc expression and progression of colorectal cancer

Mohammed A. Mansour _, Toshinori Hyodo, Khondker Ayesha Akter, Toshio Kokuryo, Keisuke Uehara, Masato Nagino and Takeshi Senga

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Oncotarget. 2016; 7:4993-5006. https://doi.org/10.18632/oncotarget.6651

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Abstract

Mohammed A. Mansour1,2, Toshinori Hyodo1, Khondker Ayesha Akter1, Toshio Kokuryo3, Keisuke Uehara3, Masato Nagino3, Takeshi Senga1

1Division of Cancer Biology, Nagoya University Graduate School of Medicine, Showa, Nagoya, 466-8550 Japan

2Biochemistry Section, Department of Chemistry, Faculty of Science, Tanta University, Tanta 31527, Egypt

3Department of Surgical Oncology, Nagoya University Graduate School of Medicine, Showa, Nagoya, 466-8550 Japan

Correspondence to:

Mohammed A. Mansour, e-mail: biomansour@hotmail.com

Keywords: colorectal cancer, SATB2, c-Myc, SATB1

Received: August 13, 2015    Accepted: December 05, 2015    Published: December 18, 2015

ABSTRACT

Special AT-rich sequence-binding protein 1 and 2 (SATB1/2) are nuclear matrix-associated proteins involved in chromatin remodeling and regulation of gene expression. SATB2 acts as a tumor suppressor in laryngeal squamous cell carcinoma and colon cancer, whereas SATB1 promotes the progression of numerous types of cancers. In this study, we examined the effects of SATB1 and SATB2 on the malignant characteristics of colorectal cancer cells. SATB1 and SATB2 expression were negatively correlated in colorectal cancer specimens. SATB1 expression was increased, whereas SATB2 expression was reduced, in colorectal cancer tissues compared to control tissues. Exogenous expression of SATB2 in colorectal cancer cells suppressed cell proliferation, colony formation and tumor proliferation in mice. c-Myc was reduced by SATB2 expression, and exogenous expression of c-Myc in SATB2-expressing cells restored proliferation, colony formation and in vivo tumor growth of colorectal cancer cells. We also showed that c-Myc reduction by SATB2 was mediated by the inactivation of ERK5. In contrast, SATB1 promoted c-Myc expression. The expression of SATB1 in colorectal cancer tissues was positively correlated with c-Myc expression, and SATB1 knockdown reduced c-Myc expression in colorectal cancer cells. Finally, we showed that SATB1 knockdown in colorectal cancer cells suppressed cell proliferation, colony formation and cell invasion. Our results reveal interesting features of how the structural homologs SATB1 and SATB2 exert opposing functions in colorectal tumorigenesis.


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