Oncotarget

Research Papers: Immunology:

Toxic effect of NiCl2 on development of the bursa of Fabricius in broiler chickens

Shuang Yin, Hengmin Cui, Xi Peng, Jing Fang, Zhicai Zuo, Junliang Deng, Xun Wang, Bangyuan Wu and Hongrui Guo _

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Oncotarget. 2016; 7:125-139. https://doi.org/10.18632/oncotarget.6591

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Abstract

Shuang Yin1, Hengmin Cui1,2, Xi Peng1,2, Jing Fang1,2, Zhicai Zuo1,2, Junliang Deng1,2, Xun Wang1,2, Bangyuan Wu1 and Hongrui Guo1

1 Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, Ya’an, Sichuan, China

2 College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, Sichuan, China

Correspondence to:

Hengmin Cui, email:

Keywords: NiCl2, lesion, relative weight, cell cycle, apoptosis, Immunology and Microbiology Section, Immune response, Immunity

Received: September 22, 2015 Accepted: November 28, 2015 Published: December 13, 2015

Abstract

This study was conducted with objective of evaluating the toxic effects of nickel chloride (NiCl2) on development of bursa of Fabricius in broilers fed on diets supplemented with 0, 300, 600 and 900 mg/kg of NiCl2 for 42 days by using the methods of experimental pathology, flow cytometry (FCM), and quantitative real-time polymerase chain reaction (qRT-PCR). The results showed that dietary NiCl2 in 300 mg/kg and over induced toxic suppression in the bursal development, which was characterized by decreasing lymphocytes histopathologically and relative weight, increasing G0/G1 phase (a prolonged nondividing state), reducing S phase (DNA replication) and proliferating index, and increasing percentages of apoptotic cells. Concurrently, the mRNA expression levels of bax, cytochrome c (cyt c), apoptotic peptidase activating factor 1 (Apaf-1), caspase-3, caspase-6, caspase-7 and caspase-9 were increased and the bcl-2 mRNA expression levels were decreased. The toxic suppression of bursal development finally impaired humoral immunity duo to the reduction of B lymphocyte population and B lymphocyte activity in the broiler chicken. This study provides new evidences for further studying the effect mechanism of Ni and Ni compoundson B-cell or bursa of Fabricius.


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