Oncotarget

Research Papers:

Targeting oncogenic PLCE1 by miR-145 impairs tumor proliferation and metastasis of esophageal squamous cell carcinoma

Xiao-Bin Cui, Su Li, Ting-Ting Li, Hao Peng, Ting-Ting Jin, Shu-Mao Zhang, Chun-Xia Liu, Lan Yang, Yao-Yuan Shen, Shu-Gang Li, Na Li, Yong Li, Jian-Ming Hu, Jin-Fang Jiang, Jing Suo, Yan Qi, Wei-Hua Liang, Liang-Hai Wang, Hong-Wei Dang, Li Li, Wei-Wei Cao, Yutao Wei, Laibo -Yin, Chuan-Yue Wu, Xiang-Lin Yuan, Hong Zhou, Yu Zheng, Yun-Zhao Chen and Feng Li _

PDF  |  HTML  |  Supplementary Files  |  How to cite  |  Order a Reprint

Oncotarget. 2016; 7:1777-1795. https://doi.org/10.18632/oncotarget.6499

Metrics: PDF 1152 views  |   HTML 1559 views  |   ?  


Abstract

Xiao-Bin Cui1,2,*, Su Li1,3,*, Ting-Ting Li1,*, Hao Peng1, Ting-Ting Jin1, Shu-Mao Zhang1, Chun-Xia Liu1, Lan Yang1, Yao-Yuan Shen4, Shu-Gang Li1, Na Li5, Yong Li6, Jian-Ming Hu1, Jin-Fang Jiang1, Jing Suo1, Yan Qi1, Wei-Hua Liang1, Liang-Hai Wang1, Hong-Wei Dang1, Li Li1, Wei-Wei Cao7, Yutao Wei8, Laibo-Yin8, Chuan-Yue Wu1,9, Xiang-Lin Yuan2, Hong Zhou10, Yu Zheng10, Yun-Zhao Chen1, Feng Li1,2

1Department of Pathology and Key Laboratory for Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine, Shihezi, China

2Department of Oncology, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, China

3Department of Pathology, Fenyang College, Shanxi Medical University, Fenyang, China

4Department of Pathology, People Hospital of Xinjiang Uygur Autonomous Region, Urumqi, China

5Department of Oncology, The First Affiliated Hospital, Shihezi University School of Medicine, Shihezi, China

6Department of CT and MRI, The First Affiliated Hospital, Shihezi University School of Medicine, Shihezi, China

7The Key Laboratory of Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine, Shihezi, China

8Department of Thoracic and Cardiovascular Surgery, The First Affiliated Hospital, Shihezi University School of Medicine, Shihezi, China

9Department of Pathology, University of Pittsburgh, Pittsburgh, PA, USA

10Bone Research Program, ANZAC Research Institute, University of Sydney, New South Wales, Australia

*These authors contributed equally to this work

Correspondence to:

Feng Li, e-mail: lifeng7855@126.com

Yunzhao Chen, e-mail: cyz0515@sina.com

Keywords: PLCE1, miR-145, esophageal carcinoma, proliferation, invasion

Received: October 23, 2015     Accepted: October 29, 2015     Published: December 08, 2015

ABSTRACT

Phospholipase C epsilon 1 (PLCE1) is a susceptibility gene in esophageal squamous cell carcinoma (ESCC). Nevertheless, the role of PLCE1 in ESCC tumorigenesis has not been elucidated. In this study, we determined the function of PLCE1 and its regulatory microRNA (miRNA) in ESCC. PLCE1 protein was excessively expressed in ESCC and precancerous lesions compared with that in normal tissues. High PLCE1 expression levels in ESCC were significantly linked with poor overall survival. Knockdown of PLCE1 promoted the apoptosis, cytokine-induced apoptosis, and sensitivity of cancer cells to chemotherapeutic drugs but abrogated the proliferation and EMT phenotype of ESCC in vitro. Notably, miR-145 was newly identified as a potent repressor of PLCE1 expression by directly targeting the 3′UTR of PLCE1. MiR-145 also inhibited cell proliferation, migration, and metastasis, as well as controlled the cytoskeleton dynamics of esophageal cancer. Moreover, miR-145 was expressed at low levels in a large cohort of patients with ESCC and was inversely correlated with PLCE1 protein expression in cancer cells and tissues. These findings demonstrate that PLCE1 functions as tumor promoter in ESCC and can be suppressed by miR-145 through inhibition of PLCE1 translation. Hence, delivery of PLCE1-targeting miR-145 is a potential therapeutic approach for esophageal cancer.


Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.
PII: 6499