Oncotarget

Research Papers:

Dynamic reprogramming of DNA methylation in SETD2-deregulated renal cell carcinoma

Rochelle L. Tiedemann, Ryan A. Hlady, Paul D. Hanavan, Douglas F. Lake, Raoul Tibes, Jeong-Heon Lee, Jeong-Hyeon Choi, Thai H. Ho and Keith D. Robertson _

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Oncotarget. 2016; 7:1927-1946. https://doi.org/10.18632/oncotarget.6481

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Abstract

Rochelle L. Tiedemann1, Ryan A. Hlady2, Paul D. Hanavan3, Douglas F. Lake3, Raoul Tibes4,5, Jeong-Heon Lee5,6, Jeong-Hyeon Choi7, Thai H. Ho4,5 and Keith D. Robertson2,5

1 Center for Epigenetics, Van Andel Research Institute, Grand Rapids, MI, USA

2 Department of Molecular Pharmacology and Experimental Therapeutics and Mayo Clinic Comprehensive Cancer Center, Mayo Clinic, Rochester, MN, USA

3 School of Life Sciences, Mayo Clinic Collaborative Research Building, Arizona State University, Scottsdale, AZ, USA

4 Division of Hematology and Medical Oncology, Mayo Clinic, Scottsdale, AZ, USA

5 Epigenomics Translational Program, Center for Individualized Medicine, Rochester, MN, USA

6 Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN, USA

7 Department of Biostatistics, Georgia Regents University, Augusta, GA, USA

Correspondence to:

Thai H. Ho, email:

Keith D. Robertson, email:

Keywords: DNA methylation, epigenetics, SETD2, histone methylation, renal cell cancer

Received: September 08, 2015 Accepted: November 16, 2015 Published: December 05, 2015

Abstract

Clear cell renal cell carcinomas (ccRCCs) harbor frequent mutations in epigenetic modifiers including SETD2, the H3K36me3 writer. We profiled DNA methylation (5mC) across the genome in cell line-based models of SETD2 inactivation and SETD2 mutant primary tumors because 5mC has been linked to H3K36me3 and is therapeutically targetable. SETD2 depleted cell line models (long-term and acute) exhibited a DNA hypermethylation phenotype coinciding with ectopic gains in H3K36me3 centered across intergenic regions adjacent to low expressing genes, which became upregulated upon dysregulation of the epigenome. Poised enhancers of developmental genes were prominent hypermethylation targets. SETD2 mutant primary ccRCCs, papillary renal cell carcinomas, and lung adenocarcinomas all demonstrated a DNA hypermethylation phenotype that segregated tumors by SETD2 genotype and advanced grade. These findings collectively demonstrate that SETD2 mutations drive tumorigenesis by coordinated disruption of the epigenome and transcriptome,and they have important implications for future therapeutic strategies targeting chromatin regulator mutant tumors.


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