RNA-binding protein CELF1 promotes tumor growth and alters gene expression in oral squamous cell carcinoma
Metrics: PDF 2620 views | HTML 2783 views | ?
Reniqua P. House1, Sudha Talwar1, E. Starr Hazard2, Elizabeth G. Hill3 and Viswanathan Palanisamy1
1 Department of Oral Health Sciences and Center for Oral Health Research, College of Dental Medicine, Medical University of South Carolina, Charleston, SC, USA
2 Division of Bioinformatics, Medical University of South Carolina, Charleston, SC, USA
3 Department of Public Health Sciences, Medical University of South Carolina, Charleston, SC, USA
Viswanathan Palanisamy, email:
Keywords: oral squamous cell carcinoma, CELF1, mRNA splicing and mRNA turnover
Received: April 15, 2015 Accepted: October 09, 2015 Published: October 20, 2015
The RNA binding protein CELF1 (also known as CUGBP1) is emerging as a critical regulator of cancer cell proliferation and apoptosis. Here, to provide a global prospective of CELF1 regulation of oral squamous cell carcinoma, we performed RNA-sequencing in oral cancer cells and CELF1 overexpression analysis in non-malignant human oral keratinocytes. Our approaches identified 1283 mRNAs differentially regulated as a function of CELF1 expression and more importantly CELF1 promoted alternative splicing of several target pre-mRNAs, which are known to be involved in various cancer biological processes. Overexpression of CELF1 in non-malignant human oral keratinocytes protected cells against oxidative damage and altered gene expression patterns. Finally, we provide evidence that reduction of CELF1 protein using a xenograft tumorigenesis mouse model decreased tumor growth. Altogether, these data provided a comprehensive view of the CELF1 mRNA regulatory network in oral cancer and suggests that CELF1 and/or its target mRNAs are viable candidates for therapeutic intervention.
All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 License.