Oncotarget

Research Papers:

Tristetraprolin induces cell cycle arrest in breast tumor cells through targeting AP-1/c-Jun and NF-κB pathway

Li Xu, Huan Ning, Ling Gu, Qinghong Wang, Wenbao Lu, Hui Peng, Weiguang Cui, Baoling Ying, Christina R. Ross, Gerald M. Wilson, Lin Wei, William S.M. Wold and Jianguo Liu _

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Oncotarget. 2015; 6:41679-41691. https://doi.org/10.18632/oncotarget.6149

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Abstract

Li Xu1,2,*, Huan Ning2,*, Ling Gu2, Qinghong Wang2, Wenbao Lu2, Hui Peng2, Weiguang Cui2, Baoling Ying3, Christina R. Ross4, Gerald M. Wilson4, Lin Wei5, William S.M. Wold3 and Jianguo Liu2

1 Department of Respiratory Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China

2 Division of Infectious Diseases, Allergy and Immunology, Department of Internal Medicine, Saint Louis University School of Medicine, St. Louis, MO, USA

3 Department of Molecular Microbiology and Immunology, Saint Louis University School of Medicine, St. Louis, MO, USA

4 Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD, USA

5 Department of Immunology, School of Basic Medicine, Hebei Medical University, Shijiazhuang, Hebei, China

* These authors have contributed equally to the work

Correspondence to:

Jianguo Liu, email:

Keywords: breast cancer, tristetraprolin, cell cycle, AP-1, apoptosis

Received: July 08, 2015 Accepted: September 30, 2015 Published: October 19, 2015

Abstract

The main characteristic of cancers, including breast cancer, is the ability of cancer cells to proliferate uncontrollably. However, the underlying mechanisms of cancer cell proliferation, especially those regulated by the RNA binding protein tristetraprolin (TTP), are not completely understood. In this study, we found that TTP inhibits cell proliferation in vitro and suppresses tumor growth in vivo through inducing cell cycle arrest at the S phase. Our studies demonstrate that TTP inhibits c-Jun expression through the C-terminal Zn finger and therefore increases Wee1 expression, a regulatory molecule which controls cell cycle transition from the S to the G2 phase. In contrast to the well-known function of TTP in regulating mRNA stability, TTP inhibits c-Jun expression at the level of transcription by selectively blocking NF-κB p65 nuclear translocation. Reconstitution of NF-κB p65 completely abolishes the inhibition of c-Jun transcription by TTP. Moreover, reconstitution of c-Jun in TTP-expressing breast tumor cells diminishes Wee1 overexpression and promotes cell proliferation. Our results indicate that TTP suppresses c-Jun expression that results in Wee1 induction which causes cell cycle arrest at the S phase and inhibition of cell proliferation. Our study provides a new pathway for TTP function as a tumor suppressor which could be targeted in tumor treatment.


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