Germ-line variants identified by next generation sequencing in a panel of estrogen and cancer associated genes correlate with poor clinical outcome in Lynch syndrome patients
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Balazs Jóri1,2, Rick Kamps4,*, Sofia Xanthoulea1,*, Bert Delvoux1, Marinus J. Blok2, Koen K. Van de Vijver3,6, Bart de Koning2, Felicia Trups Oei2, Carli M. Tops5, Ernst J. M. Speel3, Roy F. Kruitwagen1, Encarna B. Gomez-Garcia2, Andrea Romano1
1Department of Gynecology and Obstetrics, GROW – School for Oncology & Developmental Biology, Maastricht University Medical Centre, The Netherlands
2Department of Clinical Genetics, GROW – School for Oncology & Developmental Biology, Maastricht University Medical Centre, The Netherlands
3Department of Pathology, GROW – School for Oncology & Developmental Biology, Maastricht University Medical Centre, The Netherlands
4Department of Clinical Genetics, Genomics & Bioinformatics, CARIM – School for Cardiovascular Diseases, Maastricht University Medical Centre, The Netherlands
5Department of Clinical Genetics, Leiden University Medical Centre, The Netherlands
6Current address: Divisions of Diagnostic Oncology & Molecular Pathology, Netherlands Cancer Institute-Antoni van Leeuwenhoek, Amsterdam, The Netherlands
*These authors have contributed equally to this work
Andrea Romano, e-mail: [email protected]
Keywords: Lynch syndrome, endometrial cancer, genetic risk modifier, next generation sequencing, estrogens
Received: June 18, 2015 Accepted: October 09, 2015 Published: October 22, 2015
Background: The risk to develop colorectal and endometrial cancers among subjects testing positive for a pathogenic Lynch syndrome mutation varies, making the risk prediction difficult. Genetic risk modifiers alter the risk conferred by inherited Lynch syndrome mutations, and their identification can improve genetic counseling. We aimed at identifying rare genetic modifiers of the risk of Lynch syndrome endometrial cancer.
Methods: A family based approach was used to assess the presence of genetic risk modifiers among 35 Lynch syndrome mutation carriers having either a poor clinical phenotype (early age of endometrial cancer diagnosis or multiple cancers) or a neutral clinical phenotype. Putative genetic risk modifiers were identified by Next Generation Sequencing among a panel of 154 genes involved in endometrial physiology and carcinogenesis.
Results: A simple pipeline, based on an allele frequency lower than 0.001 and on predicted non-conservative amino-acid substitutions returned 54 variants that were considered putative risk modifiers. The presence of two or more risk modifying variants in women carrying a pathogenic Lynch syndrome mutation was associated with a poor clinical phenotype.
Conclusion: A gene-panel is proposed that comprehends genes that can carry variants with putative modifying effects on the risk of Lynch syndrome endometrial cancer. Validation in further studies is warranted before considering the possible use of this tool in genetic counseling.
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