Oncotarget

Research Papers:

SIP1 is a downstream effector of GADD45G in senescence induction and growth inhibition of liver tumor cells

Guiqin Xu, Li Zhang, Aihui Ma, Yu Qian, Qi Ding, Yun Liu, Boshi Wang, Zhaojuan Yang and Yongzhong Liu _

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Oncotarget. 2015; 6:33636-33647. https://doi.org/10.18632/oncotarget.5602

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Abstract

Guiqin Xu1, Li Zhang1, Aihui Ma1, Yu Qian1, Qi Ding1, Yun Liu1, Boshi Wang1, Zhaojuan Yang1 and Yongzhong Liu1

1 State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China

Correspondence to:

Yongzhong Liu, email:

Keywords: GADD45G, SIP1, cell senescence, hepatocellular carcinoma

Received: March 31, 2015 Accepted: August 26, 2015 Published: September 10, 2015

Abstract

Cellular senescence evasion caused by the inactivation of tumor suppressive programs is implicated in tumor initiation and therapeutic resistance. Our previous study has shown that the downregulation of growth arrest and DNA damage 45G (GADD45G) contributes to senescence bypass in hepatocellular carcinoma (HCC). Here, we report that the Smad-interacting protein-1 (SIP1) is transcriptionally activated and functions critically in the GADD45G-induced tumor cell senescence. Knockdown of SIP1 significantly abrogates the suppressive effects of GADD45G on the growth of xenografted liver tumor in vivo. The essential role of SIP1 in GADD45G activities is further validated in the model of the proteasome inhibitor MG132-induced cell senescence. We further show that JNK but not p38 MAPK activation is involved in the GADD45G-mediated SIP1 upregulation, and that JNK inhibition counteracts the GADD45G-induced cellular senescence. More importantly, we show that GADD45G and SIP1 expression are coincidently downregulated in primary human HCC tissues. Together, our results establish that the dowregulation of GADD45G-SIP1 axis may contribute to cellular senescence evasion and HCC development.


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