Oncotarget

Research Papers:

LYAR promotes colorectal cancer cell mobility by activating galectin-1 expression

Yupeng Wu _, Ming Liu, Zhuchen Li, Xiao-Bin Wu, Ying Wang, Yadong Wang, Min Nie, Feifei Huang, Junyi Ju, Chi Ma, Renxiang Tan, Ke Zen, Chen-Yu Zhang, Keqin Fu, Yu-Gen Chen, Ming-Rong Wang and Quan Zhao

PDF  |  HTML  |  Supplementary Files  |  How to cite  |  Order a Reprint

Oncotarget. 2015; 6:32890-32901. https://doi.org/10.18632/oncotarget.5335

Metrics: PDF 1538 views  |   HTML 1419 views  |   ?  


Abstract

Yupeng Wu1,2,*, Ming Liu1,*, Zhuchen Li1, Xiao-Bin Wu3, Ying Wang1, Yadong Wang1, Min Nie1, Feifei Huang1, Junyi Ju1, Chi Ma1, Renxiang Tan1, Ke Zen1, Chen-Yu Zhang1, Keqin Fu2, Yu-Gen Chen3, Ming-Rong Wang4, Quan Zhao1

1The State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210046, China

2Anhui Research Institute for Family Planning, Anhui Research Center for Population and Birth Control, Hefei, 230031, China

3The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, China

4The State Key Laboratory of Molecular Oncology, Cancer Hospital and Institute, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, 100021, China

*These authors have contributed equally to this work

Correspondence to:

Quan Zhao, e-mail: qzhao@nju.edu.cn

Ming-Rong Wang, e-mail: wangmr2015@126.com

Keywords: LYAR, galectin-1, colorectal cancer, cell migration and invasion

Received: May 21, 2015     Accepted: September 14, 2015     Published: September 25, 2015

ABSTRACT

Colorectal cancer (CRC) is one of the leading causes of cancer-related death worldwide. However, the molecular mechanisms of CRC pathogenesis are not fully understood. In this study, we report the characterization of LYAR (Ly-1 antibody reactive clone) as a key regulator of the migration and invasion of human CRC cells. Immunohistochemistry analysis demonstrated that LYAR is expressed at a higher level in metastatic CRC tissues. We found that LYAR promoted the migratory and invasive capabilities of CRC cells. Gene expression profile analysis of CRC cells showed that LGALS1, which encodes the galectin-1 protein, was a potential target of LYAR. The ChIP assay and gene reporter assays indicated that LYAR directly bound to the LGALS1 promoter. The ectopic expression of galectin-1 partially restored the mobile potential of LYAR knocked-down cells, which suggests that galectin-1 contributed to the LYAR-promoted cell migration and invasion of CRC cells. Thus, this study revealed a novel mechanism by which the transcription factor LYAR may promote tumor cell migration and invasion by upregulating galectin-1 gene expression in CRC.


Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.
PII: 5335