Capsaicin triggers immunogenic PEL cell death, stimulates DCs and reverts PEL-induced immune suppression
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Marisa Granato1, Maria Saveria Gilardini Montani1, Mariarosari Filardi1, Alberto Faggioni1,*, Mara Cirone1,*
1Department of Experimental Medicine, Sapienza University of Rome. 00161 Rome, Italy
Mara Cirone, e-mail: email@example.com
Alberto Faggioni, e-mail: firstname.lastname@example.org
Keywords: capsaicin, Immunogenic cell death, STAT3, DCs, PEL
Received: June 05, 2015 Accepted: August 07, 2015 Published: August 18, 2015
Capsaicin, the pungent alkaloid of red pepper has been extensively studied for its many properties, especially the anti-inflammatory and anti-oxidant ones. It binds to vanilloid receptor 1, although it has been reported to be able to mediate some effects independently of its receptor. Another important property of Capsaicin is the anticancer activity against highly malignant tumors, alone or in combination with other chemotherapeutic agents. In this study, we found that Capsaicin induced an apoptotic cell death in PEL cells correlated with the inhibition of STAT3. STAT3 pathway, constitutively activated in PEL cells, is essential for their survival. By STAT3 de-phosphorylation, Capsaicin reduced the Mcl-1 expression level and this could represent one of the underlying mechanisms leading to the Capsaicin-mediated cell death and autophagy induction. Next, by pharmacological or genetic inhibition, we found that autophagy played a pro-survival role, suggesting that its inhibition could be exploited to increase the Capsaicin cytotoxic effect against PEL cells. Finally, we show that Capsaicin induced DAMP exposure, as for an immunogenic cell death, directly promoted DC activation and, more importantly, that it counteracted the immune-suppression, in terms of DC differentiation, mediated by the PEL released factors.
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