Oncotarget

Research Papers:

Dexamethasone-induced cell death is restricted to specific molecular subgroups of multiple myeloma

Charlotte Kervoëlen, Emmanuelle Ménoret, Patricia Gomez-Bougie, Régis Bataille, Catherine Godon, Séverine Marionneau-Lambot, Philippe Moreau, Catherine Pellat-Deceunynck and Martine Amiot _

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Oncotarget. 2015; 6:26922-26934. https://doi.org/10.18632/oncotarget.4616

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Abstract

Charlotte Kervoëlen1,2, Emmanuelle Ménoret1, Patricia Gomez-Bougie2,3, Régis Bataille2, Catherine Godon4, Séverine Marionneau-Lambot5, Philippe Moreau2,3, Catherine Pellat-Deceunynck2,3, Martine Amiot2,3

1Myelomax, Nantes, France

2CRCNA, University of Nantes, INSERM, UMR892, CNRS, UMR 6299, Nantes, France

3Hematology Clinic, University Hospital, Nantes, France

4Hematology Laboratory, University Hospital, Nantes, France

5Plate-forme in vivo, Cancéropôle Grand Ouest, Nantes, France

Correspondence to:

Martine Amiot, e-mail: martine.amiot@inserm.fr

Keywords: multiple myeloma, glucocorticoid, glucocorticoid receptor, GILZ, Bim

Received: March 27, 2015     Accepted: July 06, 2015     Published: July 16, 2015

ABSTRACT

Due to its cytotoxic effect in lymphoid cells, dexamethasone is widely used in the treatment of multiple myeloma (MM). However, only a subset of myeloma patients responds to high-dose dexamethasone. Despite the undeniable anti-myeloma benefits of dexamethasone, significant adverse effects have been reported. We re-evaluate the anti-tumor effect of dexamethasone according to the molecular heterogeneity of MM. We demonstrated that the pro-death effect of dexamethasone is related to the genetic heterogeneity of MM because sensitive cell lines were restricted to MAF and MMSET signature subgroups, whereas all CCND1 cell lines (n = 10) were resistant to dexamethasone. We demonstrated that the glucocorticoid receptor expression was an important limiting factor for dexamethasone-induced cell death and we found a correlation between glucocorticoid receptor levels and the induction of glucocorticoid-induced leucine zipper (GILZ) under dexamethasone treatment. By silencing GILZ, we next demonstrated that GILZ is necessary for Dex induced apoptosis while triggering an imbalance between anti- and pro-apoptotic Bcl-2 proteins. Finally, the heterogeneity of the dexamethasone response was further confirmed in vivo using myeloma xenograft models. Our findings suggested that the effect of dexamethasone should be re-evaluated within molecular subgroups of myeloma patients to improve its efficacy and reduce its adverse effects.


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