Oncotarget

Research Papers:

MicroRNA-494 inhibits cell proliferation and invasion of chondrosarcoma cells in vivo and in vitro by directly targeting SOX9

Jingyuan Li, Lijuan Wang, Zongzhi Liu, Chao Zu, Fanfan Xing, Pei Yang, Yongkang Yang _, Xiaoqian Dang and Kunzheng Wang

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Oncotarget. 2015; 6:26216-26229. https://doi.org/10.18632/oncotarget.4460

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Abstract

Jingyuan Li1,2,*, Lijuan Wang3,*, Zongzhi Liu2, Chao Zu4, Fanfan Xing5, Pei Yang1, Yongkang Yang6, Xiaoqian Dang1, Kunzheng Wang1

1Department of Orthopaedics, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, 710004, Shaanxi Province, P.R. China

2Department of Orthopaedics, Shaanxi Provincial People’s Hospital, The Third Affiliated Hospital of Xi’an Jiaotong University, Xi’an, 710068, Shaanxi Province, P.R. China

3Department of Oncology, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, 710061, Shaanxi Province, P.R. China

4Department of Surgical Oncology, Shaanxi Provincial People’s Hospital, The Third Affiliated Hospital of Xi’an Jiaotong University, Xi’an, 710068, Shaanxi Province, P.R. China

5Department of Clinical Microbiology and Infection Control, The University of Hong Kong - Shenzhen Hospital, Shenzhen, 518053, Guangdong Province, P.R. China

6Department of Gynecology and Obstetrics, The Second Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang, 712000, Shaanxi Province, P.R. China

*These authors have contributed equally to this work

Correspondence to:

Kunzheng Wang, e-mail: [email protected]

Keywords: miR-494, chondrosarcoma, metastasis, prognosis, SOX9

Received: April 02, 2015     Accepted: June 19, 2015     Published: July 01, 2015

ABSTRACT

Accumulating evidence indicates that dysregulation of miRNAs could contribute to tumor growth and metastasis of chondrosarcoma by infuencing cell proliferation and invasion. In the current study, we are interested to examine the role of miRNAs in the carcinogenesis and progression of chondrosarcoma. Here, using comparative miRNA profiling of tissues and cells of chondrosarcoma and cartilage, we identified miR-494 as a commonly downregulated miRNA in the tissues of patients with chondrosarcoma and chondrosarcoma cancer cell line, and upregulation of miR-494 could inhibit proliferation and invasion of chondrosarcoma cancer cells in vivo and in vitro. Moreover, our data demonstrated that SOX9, the essential regulator of the process of cartilage differentiation, was the direct target and functional mediator of miR-494 in chondrosarcoma cells. And downregulation of SOX9 could also inhibit migration and invasion of chondrosarcoma cells. In the last, we identified low expression of miR-494 was significantly correlated with poor overall survival and prognosis of chondrosarcoma patients. Thus, miR-494 may be a new common therapeutic target and prognosis biomarker for chondrosarcoma.


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