Oncotarget

Research Papers:

Identification of novel long non-coding RNAs in triple-negative breast cancer

Xiaokun Shen, Bojian Xie, Zhaosheng Ma, Wenjie Yu, Wenmin Wang, Dong Xu, Xinqiang Yan, Beibei Chen, Longyao Yu, Jicheng Li, Xiaobing Chen, Kan Ding and Feilin Cao _

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Oncotarget. 2015; 6:21730-21739. https://doi.org/10.18632/oncotarget.4419

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Abstract

Xiaokun Shen1,2,*, Bojian Xie1,*, Zhaosheng Ma1,*, Wenjie Yu1, Wenmin Wang1, Dong Xu1, Xinqiang Yan1, Beibei Chen3, Longyao Yu4, Jicheng Li2, Xiaobing Chen3, Kan Ding4 and Feilin Cao1

1 Department of Surgical Oncology, Taizhou Hospital, Wenzhou Medical University, Taizhou, Zhejiang, China

2 Institute of Cell Biology, Zhejiang University, Hangzhou, China

3 Department of Internal Oncology, Hennan Cancer Hospital, The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, Hennan, China

4 Glycobiology and Glycochemistry Lab, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China

* These authors have contributed equally to this work

Correspondence to:

Xiaobing Chen, email:

Kan Ding, email:

Feilin Cao, email:

Keywords: triple-negative breast carcinomas; long non-coding RNAs

Received: January 06, 2015 Accepted: May 02, 2015 Published: June 10, 2015

Abstract

Triple-negative breast carcinomas (TNBC) are characterized by particularly poor outcomes, and there are no established markers significantly associated with prognosis. Long non-coding RNAs (lncRNAs) are subclass of noncoding RNAs that have been recently shown to play critical roles in cancer biology. However, little is known about their mechanistic role in TNBC pathogenesis. In this report, we investigated the expression patterns of lncRNAs from TNBC tissues and matched normal tissues with Agilent Human lncRNA array. We identified 1,758 lncRNAs and 1,254 mRNAs that were differentially expressed (≥ 2-fold change), indicating that many lncRNAs are significantly upregulated or downregulated in TNBC. Among these, XR_250621.1 and NONHSAT125629 were the most upregulated and downregulated lncRNAs respectively. qRT-PCR was employed to validate the microarray analysis findings, and results were consistent with the data from the microarrays. GO and KEGG pathway analysis were applied to explore the potential lncRNAs functions, some pathways including microtubule motor activity and DNA replication were identified in TNBC pathogenesis. Our study revealed that a set of lncRNAs were differentially expressed in TNBC tissues, suggesting that they may play role in TNBC. These results shed light on lncRNAs’ biological functions and provide useful information for exploring potential therapeutic targets for breast cancer.


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