Research Papers:
A pancreatic tumor-specific biomarker characterized in humans and mice as an immunogenic onco-glycoprotein is efficient in dendritic cell vaccination
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Abstract
Aurélie Collignon1,2, Adriana Teodora Perles-Barbacaru3, Stéphane Robert4,5, Françoise Silvy1,2, Emmanuelle Martinez1, Isabelle Crenon1, Sébastien Germain1,2, Stéphane Garcia6,7, Angèle Viola3, Dominique Lombardo1,2, Eric Mas1,2,* and Evelyne Béraud1,2,*
1 Aix-Marseille Université, CRO2, Centre de Recherche en Oncologie biologique et Oncopharmacologie, Marseille, France
2 Inserm, UMR_S 911, Marseille, France
3 Aix-Marseille Université, CNRS, CRMBM, Centre de Résonance Magnétique Biologique et Médicale, UMR 7339, Marseille, France
4 Aix-Marseille Université, VRCM, Vascular Research Center of Marseilles, Marseille, France
5 Inserm, UMR_S_1076, Marseille, France
6 APHM, Hôpital Nord, Laboratoire d’Anatomie-Pathologie, Marseille, France
7 Aix-Marseille Université, Marseille, France
* These authors have contributed equally to this work
Correspondence to:
Evelyne Béraud, email:
Keywords: active immunotherapy; biomarker; cancer vaccines; pancreatic cancer; tumor-associated antigen
Received: October 23, 2014 Accepted: May 30, 2015 Published: June 08, 2015
Abstract
Oncofetal fucose-rich glycovariants of the pathological bile salt-dependent lipase (pBSDL) appear during human pancreatic oncogenesis and are detected by themonoclonal antibody J28 (mAbJ28). We aimed to identify murine counterparts onpancreatic ductal adenocarcinoma (PDAC) cells and tissue and investigate the potential of dendritic cells (DC) loaded with this unique pancreatic tumor antigen to promote immunotherapy in preclinical trials. Pathological BSDLs purified from pancreatic juices of patients with PDAC were cleaved to generate glycosylated C-terminal moieties (C-ter) containing mAbJ28-reactive glycoepitopes. Immunoreactivity of the murine PDAC line Panc02 and tumor tissue to mAbJ28 was detected by immunohistochemistry and flow cytometry. C-ter-J28+ immunization promoted Th1-dominated immune responses. In vitro C-ter-J28+-loaded DCskewed CD3+ T-cells toward Th1 polarization. C-ter-J28+-DC-vaccinations selectively enhanced cell immunoreactivity to Panc02, as demonstrated by CD4+- and CD8+-T-cell activation, increased percentages of CD4+- and CD8+-T-cells and NK1.1+ cells expressing granzyme B, and T-cell cytotoxicity. Prophylactic and therapeutic C-ter-J28+-DC-vaccinations reduced ectopic Panc02-tumor growth, provided long-lasting protection from Panc02-tumor development in 100% of micebut not from melanoma, and attenuated progression of orthotopic tumors as revealed by MRI. Thusmurine DC loaded with pancreatic tumor-specific glycoepitope C-ter-J28+ induce efficient anticancer adaptive immunity and represent a potential adjuvant therapy for patients afflicted with PDAC.
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