Oncotarget

Research Papers:

Expression of miR-27a-3p is an independent predictive factor for recurrence in clear cell renal cell carcinoma

Wataru Nakata _, Motohide Uemura, Mototaka Sato, Kazutoshi Fujita, Kentaro Jingushi, Yuko Ueda, Kaori Kitae, Kazutake Tsujikawa and Norio Nonomura

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Oncotarget. 2015; 6:21645-21654. https://doi.org/10.18632/oncotarget.4064

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Abstract

Wataru Nakata1, Motohide Uemura1, Mototaka Sato1, Kazutoshi Fujita1, Kentaro Jingushi2, Yuko Ueda2, Kaori Kitae2, Kazutake Tsujikawa2, Norio Nonomura1

1The Department of Urology, Osaka University Graduate School of Medicine, Osaka, Japan

2Laboratory of Molecular and Cellular Physiology, Osaka University Graduate School of Pharmaceutical Sciences, Osaka, Japan

Correspondence to:

Motohide Uemura, e-mail: uemura@uro.med.osaka-u.ac.jp

Keywords: clear cell carcinoma, renal cell carcinoma, microRNA, prognosis, recurrence

Received: January 18, 2015     Accepted: May 15, 2015     Published: May 27, 2015

ABSTRACT

MicroRNAs (miRNAs) are noncoding RNAs that regulate gene expression and function in tumor development and progression. We previously identified up-regulated miRNAs in clear cell renal cell carcinoma (ccRCC) compared to matched-pair normal kidney by microarray. Here, we identify miRNAs that are up-regulated in ccRCC and are also correlated with survival and/or recurrence. Twenty-four samples from ccRCC patients who underwent nephrectomies between 2011 and 2012 were divided into two groups: one of eleven patients who experienced recurrence (Group 1), and one of thirteen patients with no evidence of disease (Group 2) 2 years after surgery. Analyzing 22 miRNAs that were up-regulated in ccRCC in our previous study, we identify five miRNAs that were statistically up-regulated in Group 1 versus Group 2 by quantitative real-time PCR. We then evaluated these miRNAs in an independent cohort of 159 frozen ccRCC samples. High levels of miR-27a-3p (p < 0.01) correlated with a worse progression-free survival rate. Multivariate analysis revealed that miR-27a-3p was an independent predictive factor for recurrence. For functional analysis, miR-27a-3p controlled cell proliferation, migration and invasion in RCC cell lines. MiR-27a-3p could act as oncogenic miRNA and may be a candidate for targeted molecular therapy in ccRCC.


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