Upregulated long non-coding RNA AFAP1-AS1 expression is associated with progression and poor prognosis of nasopharyngeal carcinoma
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Hao Bo1,2,3, Zhaojian Gong2,4, Wenling Zhang2, Xiayu Li3, Yong Zeng1, Qianjin Liao1, Pan Chen1, Lei Shi4, Yu Lian2, Yizhou Jing2, Ke Tang2, Zheng Li1,2,3, Yanhong Zhou1,2,3, Ming Zhou1,2,3, Bo Xiang1,2,3, Xiaoling Li1,2,3, Jianbo Yang1,5, Wei Xiong1,2,3, Guiyuan Li1,2,3 and Zhaoyang Zeng1,2,3
1 Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan, China
2 The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health and The Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute, Central South University, Changsha, Hunan, China
3 Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Disease Genome Research Center, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China
4 Department of Oral and Maxillofacial Surgery, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China
5 Department of Laboratory Medicine and Pathology and Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, United States of America
Zhaoyang Zeng, email:
Keywords: long non-coding RNA (LncRNA); AFAP1 antisense RNA1 (AFAP1-AS1); nasopharyngeal carcinoma (NPC); metastasis; prognosis
Received: March 10, 2015 Accepted: April 22, 2015 Published: May 09, 2015
Altered expression of long noncoding RNAs (lncRNAs) associated with human carcinogenesis. We performed a cDNA microarray analysis of lncRNA expression in 12 cases of nasopharyngeal carcinoma (NPC) and 4 non-tumor nasopharyngeal epitheliums. One lncRNA, actin filament associated protein 1 antisense RNA1 (AFAP1-AS1), was identified and selected for further study. AFAP1-AS1 expression was upregulated in NPC and associated with NPC metastasis and poor prognosis. In vitro experiments demonstrated that AFAP1-AS1 knockdown significantly inhibited the NPC cell migration and invasive capability. AFAP1-AS1 knockdown also increased AFAP1 protein expression. Proteomic and bioinformatics analyses suggested that AFAP1-AS1 affected the expression of several small GTPase family members and molecules in the actin cytokeratin signaling pathway. AFAP1-AS1 promoted cancer cell metastasis via regulation of actin filament integrity. AFAP1-AS1 might be a potential novel marker that can predict cancer patient prognosis and as a potential therapeutic target for NPC.
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