Research Papers:

MicroRNA-101 inhibits cell progression and increases paclitaxel sensitivity by suppressing MCL-1 expression in human triple-negative breast cancer

Xiaoping Liu, Hailin Tang, Jianping Chen, Cailu Song, Lu Yang, Peng Liu, Neng Wang, Xinhua Xie, Xiaoti Lin and Xiaoming Xie _

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Oncotarget. 2015; 6:20070-20083. https://doi.org/10.18632/oncotarget.4039

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Xiaoping Liu1,*, Hailin Tang1,*, Jianping Chen2, Cailu Song1, Lu Yang1, Peng Liu1, Neng Wang2, Xinhua Xie1, Xiaoti Lin1 and Xiaoming Xie1

1 Department of Breast Oncology, Sun Yat-Sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, Guangdong, China

2 School of Chinese Medicine, The University of Hong Kong, Hong Kong

* These authors have contributed equally to this work

Correspondence to:

Xiaoming Xie, email:

Keywords: miR-101, triple-negative breast cancer, paclitaxel, sensitivity, MCL-1

Received: February 28, 2015 Accepted: April 20, 2015 Published: May 08, 2015


Triple-negative breast cancer is the most aggressive breast cancer subtype. The aim of our study was to investigate the functional role of both miR-101 and MCL-1 in the sensitivity of human triple-negative breast cancer (TNBC) to paclitaxel. We found that the expression of miR-101 was strongly decreased in triple-negative breast cancer tissues and cell lines. The expression of miR-101 was not associated with clinical stage or lymph node infiltration in TNBC. Ectopic overexpression of miR-101 inhibit growth and induced apoptosis in vitro and suppressed tumorigenicity in vivo. MCL-1 was significantly overexpressed in most of the TNBC tissues and cell lines. Luciferase assay results confirmed MCL-1 as a direct target gene of miR-101. MiR-101 inhibited MCL-1 expression in TNBC cells and transplanted tumors. There was a negative correlation between the level of expression of miR-101 and MCL-1 in TNBC tissues. Suppression of MCL-1 enhanced the sensitivity of MDA-MB-435 cells to paclitaxel. Furthermore, miR-101 increased paclitaxel sensitivity by inhibiting MCL-1 expression. Our findings provide significant insight into the molecular mechanisms of TNBC carcinogenesis and may have clinical relevance for the development of novel, targeted therapies for TNBC.

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