Prognostic significance of the TREK-1 K2P potassium channels in prostate cancer
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Gui-Ming Zhang1,2, Fang-Ning Wan1,2, Xiao-Jian Qin1, Da-Long Cao1, Hai-Liang Zhang1, Yao Zhu1, Bo Dai1, Guo-Hai Shi1, Ding-Wei Ye1
1Department of Urology, Fudan University Shanghai Cancer Center, Shanghai, China
2Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China
Guo-Hai Shi, e-mail: firstname.lastname@example.org
Ding-Wei Ye, e-mail: email@example.com
Keywords: TREK-1, prostate cancer, biochemical recurrence, cell proliferation, cell cycle
Received: March 05, 2015 Accepted: April 13, 2015 Published: April 21, 2015
Background: TREK-1 channels belong to the two-pore domain potassium channel superfamily and play an important role in central nervous system diseases. However, few studies have examined their role in carcinogenesis.
Methods: In this study, we assessed the expression of TREK-1 in 100 prostate cancer (PCa) tissues using immunohistochemistry and further analyzed its clinicopathological significance. Next, cell proliferation and cell cycle analysis were carried out on human PCa PC-3 cell lines where TREK-1 was stably knockdown.
Results: We found that compared with normal prostate tissues, PCa tissues showed overexpressed TREK-1 levels and TREK-1 levels were positively associated with Gleason score and T staging. High level of TREK-1 expression was related to shorter castration resistance free survival (CRFS). Furthermore, knockdown of TREK-1 significantly inhibited PCa cell proliferation in vitro and in vivo, and induced a G1/S cell cycle arrest.
Conclusion: Our results suggest that TREK-1 might be a biomarker in CRFS judgment of PCa, as well as a potential therapeutic target.
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