Oncotarget

Research Papers:

MerTK is a novel therapeutic target in gastric cancer

Jun Ho Yi _, Jiryeon Jang, Jeong Hee Cho, In-Gu Do, Mineui Hong, Seung Tae Kim, Kyoung-Mee Kim, Sujin Lee, Se Hoon Park, Joon Oh Park, Young Suk Park, Won Ki Kang, Ho Yeong Lim and Jeeyun Lee

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Oncotarget. 2017; 8:96656-96667. https://doi.org/10.18632/oncotarget.3750

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Abstract

Jun Ho Yi1,5,*, Jiryeon Jang1,*, Jeonghee Cho2,*, In-Gu Do3, Mineui Hong4, Seung Tae Kim1, Kyoung-Mee Kim4, Sujin Lee1, Se Hoon Park1, Joon Oh Park1,4, Young Suk Park1, Won Ki Kang1, Ho Yeong Lim1 and Jeeyun Lee1

1Division of Hematology-Oncology, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea

2Samsung Genome Institute, Seoul, Korea

3Department of Pathology, Kangbuk Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea

4Department of Pathology and Translational Genomics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea

5Division of Hematology-Oncology, Department of Medicine, Hanyang University Hospital, Seoul, Korea

*These authors have contributed equally to this work

Correspondence to:

Ho Yeong Lim, e-mail: hoylim@skku.edu

Jeeyun Lee, e-mail: jyunlee@skku.edu

Keywords: gastric cancer, MerTK, patient-derived cells

Received: February 28, 2014     Accepted: April 08, 2015     Published: April 20, 2015

ABSTRACT

Introduction: The role of MerTK has not been assessed in gastric cancer (GC). The aim of this study was to identify a subgroup of GC patients with MerTK tumor overexpression, and to evaluate MerTK as a potential therapeutic target in this disease.

Methods: Protein and mRNA expression of MerTK were evaluated, and other various in vitro analyses including shRNA transfection, cell cycle anslysis, MTS assay and colony forming assay were carried out with GC cell lines and GC patient-derived cells (PDCs).

Results: shRNA-mediated knockdown of MerTK resulted in inhibition of cell growth, as well as increased cellular apoptosis in MerTK positive GC cells. Out of 192 GC patients, 16 (8.3%) patients showed strong protein expression and they had a significantly shorter overall survival compared to those with no MerTK expression. In 54 cases of GC PDCs, 4 cases (7.4%) showed mRNA overexpression, which was comparable to the protein expression rate. When we administered UNC1062, a novel MerTK-selective small molecular tyrosine kinase inhibitor, proliferation of MerTK overexpressing GC cells and PDCs were considerably inhibited.

Conclusion: MerTK may be involved in GC carcinogenesis, and it could be a potential novel therapeutic target in GC patients.


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