Oncotarget

Research Papers: Pathology:

Smad7 protects against chronic aristolochic acid nephropathy in mice

Xiao-Yu Dai, Li Zhou, Xiao-Ru Huang, Ping Fu and Hui-Yao Lan _

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Oncotarget. 2015; 6:11930-11944. https://doi.org/10.18632/oncotarget.3718

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Abstract

Xiao-Yu Dai1,2,*, Li Zhou1,2,*, Xiao-Ru Huang1, Ping Fu2 and Hui-Yao Lan1

1 Department of Medicine and Therapeutics, and Li Ka Shing Institute of Health Sciences, and Shenzhen Research Institute, The Chinese University of Hong Kong, Hong Kong, China

2 Division of Nephrology, West China Hospital of Sichuan University, Chengdu, China

* These authors have contributed equally to this work

Correspondence to:

Hui Y. Lan, email:

Ping Fu, email:

Keywords: chronic aristolochic acid nephropathy, Smad7, renal fibrosis, renal inflammation

Received: January 26, 2015 Accepted: March 05, 2015 Published: March 30, 2015

Abstract

Chronic Aristolochic Acid Nephropathy (AAN) is a progressive chronic kidney disease related to herb medicine. However, treatment for chronic AAN remains ineffective. We report here that Smad7 is protective and has therapeutic potential for chronic AAN. In a mouse model of chronic AAN, progressive renal injury was associated with a loss of renal Smad7 and disruption of Smad7 largely aggravated the severity of chronic AAN as demonstrated by a significant increase in levels of 24-hour urinary protein excretion, serum creatinine, and progressive renal fibrosis and inflammation. In contrast, restored Smad7 locally in the kidneys of Smad7 knockout mice prevented the progression of chronic AAN. Further studies revealed that worsen chronic AAN in Smad7 knockout mice was associated with enhanced activation of TGF-β/Smad3 and NF-κB signaling pathways, which was reversed when renal Smad7 was restored. Importantly, we also found that overexpression of Smad7 locally in the kidneys with established chronic AAN was capable of attenuating progressive chronic AAN by inactivating TGF-β/Smad3-medated renal fibrosis and NF-κB-driven renal inflammation. In conclusion, Smad7 plays a protective role in the pathogenesis of chronic AAN and overexpression of Smad7 may represent a novel therapeutic potential for chronic AAN.


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