TOPK is highly expressed in circulating tumor cells, enabling metastasis of prostate cancer
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Huimin Sun1,*, Lei Zhang2,*, Changhong Shi3, Peizhen Hu4, Wei Yan4, Zhe Wang4, Qiuhong Duan5, Fan Lu6, Lipeng Qin7, Tao Lu5, Juanjuan Xiao5, Yingmei Wang4, Feng Zhu5, Chen Shao1
1 Department of Urology, Xijing Hospital, The Fourth Military Medical University, Xian, China
2 Department of Epidemiology, Faculty of Preventive Medicine, The Fourth Military Medical University, Xian, China
3 Laboratory Animal Center, The Fourth Military Medical University, Xian, Shaanxi, China
4 Department of Pathology, Xijing Hospital, The Fourth Military Medical University, Xian, China
5 Department of Biochemistry and Molecular Biology, School of Basic Medicine, Huazhong University of Science and Technology, Wuhan, China
6 Department of Biochemistry, Department of Basic Medicine, The Fourth Military Medical University, Xian, China
7 PLA Lhasa General Hospital, Lhasa, China
* This authors are Joint first author
Feng Zhu, email:
Chen Shao, email:
Keywords: circulating tumor cells, T-LAK cell-originated protein kinase (TOPK), prostate cancer, metastasis
Received: December 13, 2014 Accepted: February 26, 2015 Published: March 20, 2015
Circulating tumor cells (CTCs) are important for metastasis in prostate cancer. T-LAK cell-originated protein kinase (TOPK) is highly expressed in cancer cells. Herein, we established a xenograft animal model, isolated and cultured the CTCs, and found CTCs have significantly greater migratory capacity than parental cells. TOPK is more highly expressed in the CTCs than in parental cells and is also highly expressed in the metastatic nodules caused by CTCs in mice. Knocking down TOPK decreased the migration of CTCs both in vitro and in vivo. TOPK was modulated by the PI3K/PTEN and ERK pathways during the metastasis of prostate cancer. High levels of TOPK in the tumors of patients were correlated with advanced stages of prostate cancer, especially for high-risk patients of Gleason score≥8, PSA>20ng/ml. In summary, TOPK was speculated to be one of a potential marker and therapeutic target in advanced prostate cancer.
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