Priority Research Papers:
miR-181c associates with tumor relapse of high grade osteosarcoma
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Federica Mori1, Andrea Sacconi2, Valeria Canu2, Federica Ganci2, Mariangela Novello3, Vincenzo Anelli4, Renato Covello5, Virginia Ferraresi6, Paola Muti7, Roberto Biagini8, Giovanni Blandino2,7 and Sabrina Strano1,7
1 Molecular Chemoprevention Unit, Regina Elena National Cancer Institute, Rome, Italy
2 Translational Oncogenomics, Regina Elena National Cancer Institute, Rome, Italy
3 Department of Pathology, Catholic University, Rome, Italy
4 UOC Radiology, Regina Elena National Cancer Institute, Rome, Italy
5 UOC Department of Pathology, Regina Elena National Cancer Institute, Rome, Italy
6 Medical Oncology A, Regina Elena National Cancer Institute, Rome, Italy
7 Department of Oncology, McMaster University, Hamilton, ON, Canada
8 UOC Orthopedic Surgery, Regina Elena National Cancer Institute, Rome, Italy
Giovanni Blandino, email:
Keywords: giant cell tumor, osteosarcoma, miRNA profiling, relapse
Received: December 11, 2014 Accepted: February 17, 2015 Published: March 12, 2015
High-grade osteosarcoma (OS) is characterized by low incidence, high aggressiveness and moderate 5-years survival rate after aggressive poly-chemotherapy and surgery. Here we used miRNA profiling as a tool to possibly predict and monitor OS’s development and therapeutic outcome. First, we evaluated the altered expression of selected miRNAs from a case of Giant Cell Tumor (GCT) apparently evolved into an OS. We found that most of modulated miRs were associated with pathways of bone resorption and osteogenesis. miRNA expression also revealed that GCT and OS were distinct tumors. Second, we validated the observed miRNA profile in two independent casuistries of ten GCT (not evolved into malignant tumors) and sixteen OS patients. Interestingly, we found that miR-181c and other three miRNAs identified in the first step of the study were also consistently de-regulated in all OS patients. Ectopic expression of miR-181c reduced cell viability and enhanced chemotherapeutic-induced cell death of U2OS and SAOS2 cells. These findings indicate that: i) miRNAs aberrantly modulated in GCT could be predictive of its development into OS and ii) miRNAs expression could be useful to monitor the OS therapeutic outcome.
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