Oncotarget

Research Papers:

This article has been corrected. Correction in: Oncotarget. 2017; 8(15):25830.

Amphiregulin enhances alpha6beta1 integrin expression and cell motility in human chondrosarcoma cells through Ras/Raf/MEK/ERK/AP-1 pathway

Jui-Chieh Chen, Yu-Ju Chen, Chih-Yang Lin, Yi-Chin Fong, Chin-Jung Hsu, Chun-Hao Tsai, Jen-Liang Su and Chih-Hsin Tang _

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Oncotarget. 2015; 6:11434-11446. https://doi.org/10.18632/oncotarget.3397

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Abstract

Jui-Chieh Chen1,2,3, Yu-Ju Chen4, Chih-Yang Lin2, Yi-Chin Fong5,6, Chin-Jung Hsu5,6, Chun-Hao Tsai6,7, Jen-Liang Su3,8,9,10, Chih-Hsin Tang2,8,11

1Department of Biochemical Science and Technology, National Chiayi University, Chiayi, Taiwan

2Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan

3National Institute of Cancer Research, National Health Research Institutes, Miaoli County, Taiwan

4School of Pharmacy, China Medical University, Taichung, Taiwan

5School of Chinese Medicine, College of Chinese Medicine, China Medical University, Taichung, Taiwan

6Department of Orthopedic Surgery, China Medical University Hospital, Taichung, Taiwan

7Department of Medicine and Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan

8Department of Biotechnology, College of Health Science, Asia University, Taichung, Taiwan

9Center for Molecular Medicine, China Medical University Hospital, Taichung, Taiwan

10Graduate Institute of Cancer Biology, China Medical University, Taichung, Taiwan

11Department of Pharmacology, School of Medicine, China Medical University, Taichung, Taiwan

Correspondence to:

Chih-Hsin Tang, e-mail: [email protected]

Jen-Liang Su, e-mail: [email protected]

Keywords: chondrosarcoma, amphiregulin, integrin, cell migration

Received: January 06, 2015     Accepted: February 16, 2015     Published: March 18, 2015

ABSTRACT

Chondrosarcoma is a malignant tumor that produces cartilage matrix. The most lethal aspect is its metastatic property. We demonstrated that amphiregulin (AR) is significantly upregulated in highly aggressive cells. AR silencing markedly suppressed cell migration. Exogenous AR markedly increased cell migration by transactivation of α6β1 integrin expression. A neutralizing α6β1 integrin antibody can abolish AR-induced cell motility. Knockdown of AR inhibits metastasis of cells to the lung in vivo. Furthermore, elevated AR expression is positively correlated with α6β1 integrin levels and higher grades in patients. These findings can potentially serve as biomarker and therapeutic approach for controlling chondrosarcoma metastasis.


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