Research Papers:

Cordycepin (3’-deoxyadenosine) suppressed HMGA2, Twist1 and ZEB1-dependent melanoma invasion and metastasis by targeting miR-33b

Pu Zhang _, Changjin Huang, Changliang Fu, Yang Tian, Yijuan Hu, Bochu Wang, Amy Strasner, Yang Song and Erqun Song

PDF  |  HTML  |  Supplementary Files  |  How to cite

Oncotarget. 2015; 6:9834-9853. https://doi.org/10.18632/oncotarget.3383

Metrics: PDF 3306 views  |   HTML 3362 views  |   ?  


Pu Zhang1,4, Changjin Huang2,5, Changliang Fu4, Yang Tian2,6, Yijuan Hu8, Bochu Wang3, Amy Strasner7, Yang Song1, Erqun Song1

1Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China

2Institute of Pathology, Third Military Medical University, Chongqing 400038, China

3College of Bioengineering, Chongqing University, Chongqing 400030, China

4Department of Bioengineering, Pennsylvania State University, University Park, PA 16801, USA

5Division of Hematology/Oncology, Department of Medicine, Robert H. Lurie Comprehensive Cancer Center, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA

6Department of Pathology, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA

7Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California at San Diego, La Jolla, CA 92093, USA

8Rollins School of Public Health, Emory University, Atlanta, GA 30322, USA

Correspondence to:

Pu Zhang, e-mail: [email protected]; e-mail: [email protected]

Keywords: cordycepin, miR-33b, metastasis, cell migration, focal adhesion

Received: January 20, 2015     Accepted: February 14, 2015     Published: March 13, 2015


Malignant melanoma, the most deadly form of skin cancer, has a high propensity for metastatic spread and is notoriously chemotherapy-resistant. Cordycepin, the active component of Cordyceps spp., has been identified to have anti-metastatic effect on tumor progression and thus possesses pharmacological and therapeutic potentials. However, the mechanisms of anti-metastatic effects of cordycepin at cellular levels remain elusive. We analyzed the effect of cordycepin on human melanoma miRNA expression profiles by miRNAarray and found that miR-33b was upregulated in highly-metastatic melanoma cell lines following cordycepin exposure. Cordycepin-mediated miR-33b expression was dependent on LXR-RXR heterodimer activation. miR-33b directly binds to HMGA2, Twist1 and ZEB1 3’-UTR to suppress their expression. The negative correlations between miR-33b levels and HMGA2, Twist1 or ZEB1 expression were detected in 72 patient melanoma tissue samples. By targeting HMGA2 and Twist1, miR-33b attenuated melanoma migration and invasiveness upon cordycepin exposure. miR-33b knockdown or ZEB1 overexpression reverted cordycepin-mediated mesenchymal-epithelial transition (MET), triggering the expression of N-cadherin. In spontaneous metastasis models, cordycepin suppressed tumor metastasis without altering primary tumor growth. We showed for the first time that targeting miRNA by cordycepin indicates a new mechanism of cordycepin-induced suppression of tumor metastasis and miR-33b/HMGA2/Twist1/ZEB1 axis plays critical roles in regulating melanoma dissemination.

Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 License.
PII: 3383