Clinical Research Papers:
Deregulation of dicer and mir-155 expression in liposarcoma
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Bruno Vincenzi1, Michele Iuliani2, Alice Zoccoli2, Francesco Pantano1, Marco Fioramonti2, Delia De Lisi1, Anna Maria Frezza1, Carla Rabitti3, Giuseppe Perrone3, Andrea Onetti Muda3, Antonio Russo5, Antonio Giordano6,7, Daniele Santini1, Angelo Paolo Dei Tos4, Giuseppe Tonini1
1Medical Oncology, Campus Bio-Medico University of Rome, Rome, Italy
2Translational Oncology Laboratory, Campus Bio-Medico University of Rome, Rome, Italy
3Pathology, Campus Bio-Medico University of Rome, Rome, Italy
4Anatomic Pathology, General Hospital of Treviso, Treviso, Italy
5Department of Surgical, Oncological and Oral Sciences, University of Palermo, Palermo, Italy
6Sbarro Institute for Cancer Research and Molecular Medicine, College of Science and Technology, Temple University BioLife Science, Philadelphia, PA, USA
7Department of Medicine, Surgery & Neuroscience University of Siena, Italy
Antonio Russo, e-mail: [email protected]
Keywords: Dicer, Drosha, mir-155, liposarcoma
Received: December 19, 2014 Accepted: January 23, 2015 Published: April 04, 2015
Liposarcoma (LPS) is the most common soft tissue sarcoma. It has been demonstrated that mir-155 was the most overexpressed miRNA in well-differentiated LPS(WDLPS)/dedifferentiated LPS (DDLPS). The aim of this study is to evaluate the involvement of Dicer, Drosha and mir-155 in development of LPS and their possible role in stratification of different histological subtypes. Dicer, Drosha and mir-155 mRNA levels were analyzed in formalin-fixed paraffin-embedded specimens from patients diagnosed with 62 LPS and compared with samples of adipose tissues of healthy donors. The experimental data were obtained using qRT-PCR comparing Dicer, Drosha and mir-155 expression levels in tumor samples versus normal fat. The tumor samples from LPS patients showed a significantly lower Dicer expression versus normal adipose tissue, while Drosha levels did not differ. Concerning mir155 expression levels, our results demonstrated a significant mir-155 up-regulation in all LPS subtypes versus normal adipose tissue except for WDLS. These findings demonstrate for the first time that Dicer is deregulated in LPS and show that mir-155 is differentially expressed in LPS subgroups and it could be a promising tool to improve LPS disease stratification and differential diagnosis.
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