Oncotarget

Research Papers:

MET receptor is a potential therapeutic target in high grade cervical cancer

Katarzyna Miekus, Marta Pawlowska, Małgorzata Sekuła, Grazyna Drabik, Zbigniew Madeja, Dariusz Adamek and Marcin Majka _

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Oncotarget. 2015; 6:10086-10101. https://doi.org/10.18632/oncotarget.3161

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Abstract

Katarzyna Miekus1, Marta Pawlowska2, Małgorzata Sekuła2, Grazyna Drabik2, Zbigniew Madeja4, Dariusz Adamek5, Marcin Majka2

1Department of General Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Cracow, Poland

2Department of Transplantation, Polish-American Institute of Pediatrics, Jagiellonian University Medical College, Cracow, Poland

3Department of Clinical Immunology, Polish-American Institute of Pediatrics, Jagiellonian University Medical College, Cracow, Poland

4Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Cracow, Poland

5Department of Pathomorphology, Jagiellonian University Medical College, Cracow, Poland

Correspondence to:

Marcin Majka, e-mail: [email protected]

Katarzyna Miekus, e-mail: [email protected]

Keywords: cervical cancer, MET, CXCR4, differentiation, E-cadherin

Received: December 17, 2014     Accepted: January 17, 2015     Published: April 04, 2015

ABSTRACT

Cervical cancer is one of the leading causes of death among women suffering from tumors. Current treatment options are insufficient. Here, we investigated the MET receptor as a potential molecular target in advanced cervical cancer. Downregulation of MET receptor expression via RNA interference in different cervical carcinoma cell lines dramatically decreased tumor growth and forced tumor differentiation in vivo. MET receptor silencing also led to a dramatic decrease in cell size and a decrease in proliferation rate under normal and stress conditions. MET receptor downregulation also resulted in decreased cyclin D1 and c-myc levels but did not increase apoptosis. Subsequent experiments showed that downregulation of the MET receptor decreased the expression of a key regulator of the epithelial-to-mesenchymal transition, SLUG. and increased the expression of E-cadherin, a hallmark of the epithelial phenotype. Moreover, MET downregulation impairs expression and signaling of CXCR4 receptor, responsible for invasive phenotype.

Taken together, our results strongly suggest that the MET receptor influences the oncogenic properties of cervical carcinoma cells in vitro and in vivo. These findings highlight a unique role of the MET receptor in cervical carcinoma cells and indicate the MET receptor as a potential therapeutic target for advanced cervical carcinoma.


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