Oncotarget

Research Papers:

Molecular chaperone GRP78 enhances aggresome delivery to autophagosomes to promote drug resistance in multiple myeloma

Mohamed A.Y. Abdel Malek, Sajjeev Jagannathan, Ehsan Malek, Douaa M. Sayed, Sahar A. Elgammal, Hanan G. Abd El-Azeem, Nabila M. Thabet and James J. Driscoll _

PDF  |  HTML  |  How to cite  |  Order a Reprint

Oncotarget. 2015; 6:3098-3110. https://doi.org/10.18632/oncotarget.3075

Metrics: PDF 2834 views  |   HTML 4637 views  |   ?  


Abstract

Mohamed A.Y. Abdel Malek1,2,3,*, Sajjeev Jagannathan1,2,*, Ehsan Malek1,2, Douaa M. Sayed4, Sahar A. Elgammal3, Hanan G. Abd El-Azeem3, Nabila M. Thabet3 and James J. Driscoll1,2,5,6

1 The Vontz Center for Molecular Studies, University of Cincinnati College of Medicine, Cincinnati, OH

2 Division of Hematology and Oncology, University of Cincinnati College of Medicine, Cincinnati, OH

3 Department of Clinical Pathology, Faculty of Medicine, Assiut University, Assiut, Egypt

4 Department of Clinical Pathology, South Egypt Cancer Institute, Assiut University, Assiut, Egypt

5 Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, OH

6 University of Cincinnati Cancer Institute, Cincinnati, OH

* These authors contributed equally to this work

Correspondence:

James J. Driscoll, email:

Keywords: GRP78, Aggresome+Autophagosome Pathway, Proteasome, Myeloma, Chemoresistance

Received: November 06, 2014 Accepted: December 17, 2014 Published: December 26, 2014

Abstract

Despite the clinical benefit of the proteasome inhibitor bortezomib, multiple myeloma (MM) patients invariably relapse through poorly defined mechanisms. Myeloma cells inevitably develop chemoresistance that leads to disease relapse and patient-related deaths. Studies in tumor cell lines and biopsies obtained from patients refractory to therapy have revealed that myeloma cells adapt to stress by inducing expression of glucose-regulated protein 78 (GRP78), an endoplasmic reticulum (ER) chaperone with anti-apoptotic properties. Treatment of myeloma cells with bortezomib increased GRP78 levels and activated GRP78-dependent autophagy. Expression profiling indicated that GRP78-encoding HSPA5 was significantly upregulated in bortezomib-resistant cells. Co-treatment with the anti-diabetic agent metformin suppressed GRP78 and enhanced the anti-proliferative effect of bortezomib. Bortezomib treatment led to GRP78 co-localization with proteotoxic protein aggregates, known as aggresomes. Pharmacologic suppression, genetic ablation or mutational inactivation of GRP78 followed by bortezomib treatment led to the accumulation of aggresomes but impaired autophagy and enhanced anti-myeloma effect of bortezomib. GRP78 was co-immunoprecipitated with the KDEL receptor, an ER quality control regulator that binds proteins bearing the KDEL motif to mediate their retrieval from the Golgi complex back to the ER. Taken together, we demonstrate that inhibition of GRP78 functional activity disrupts autophagy and enhances the anti-myeloma effect of bortezomib.


Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.
PII: 3075