Oncotarget

Clinical Research Papers:

Poor survival is associated with the methylated degree of Zinc-finger protein 545 (ZNF545) DNA promoter in gastric cancer

Jingyu Deng _, Han Liang, Guoguang Ying, Qiuping Dong, Rupeng Zhang, Jun Yu, Daiming Fan and Xishan Hao

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Oncotarget. 2015; 6:4482-4495. https://doi.org/10.18632/oncotarget.2916

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Abstract

Jingyu Deng1, Han Liang1, Guoguang Ying2, Qiuping Dong2, Rupeng Zhang1, Jun Yu3, Daiming Fan4, Xishan Hao1

1Department of Gastroenterology, Tianjin Medical University Cancer Hospital, City Key Laboratory of Tianjin Cancer Center and National Clinical Research Center for Cancer, Tianjin, China

2Central laboratory, Tianjin Medical University Cancer Hospital, City Key Laboratory of Tianjin Cancer Center and National Clinical Research Center for Cancer, Tianjin, China

3Institute of Digestive Disease, Li Ka Shing Institute of Health Science, Chinese University of HongKong, Shatin, HongKong

4State Key Laboratory of Cancer Biology and Institute of Digestive Diseases, Xijing Hospital, Fourth Military Medical University, Xi'an, China

Correspondence to:

Han Liang, e-mail: tjlianghan@sohu.com

Xishan Hao, e-mail: tjhaoxishan@sohu.com

Keywords: stomach, neoplasm, zinc-finger protein, survival, methylation

Received: November 16, 2014     Accepted: December 11, 2014     Published: February 26, 2015

ABSTRACT

Zinc-finger protein 545 (ZNF545) was identified as a gastric tumour suppressor and potentially independent prognostic factor. At the present study, we found that lower expression of ZNF545 was specific in gastric cancer (GC) tissues, and the inconsistently methylated levels of ZNF545 promoter were identified in the gastric cancer tissues. In the methylation-specific PCR (MSP) analysis cohort, we found that GC patients with hypermethylated ZNF545 promoter exhibited significantly shorter median OS than those with unmethylated ZNF545 promoter and those with hypomethylated ZNF545 promoter. In the other cohort, we also demonstrated that GC patients with three or more methylated CpG sites in the ZNF545 promoter were significantly associated with poor survival by using the bisulphite gene sequencing (BGS). The methylated degrees of five CpG sites (−232, −214, −176, −144 and −116) could also provide distinct survival discrimination of patients with GC. These findings indicated that the methylated CpG sites of the ZNF545 promoter could be used for the clinical prediction of the prognosis of GC.


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