Correction: β2-spectrin depletion impairs DNA damage repair

Nobuo Horikoshi¹,², Raj K. Pandita¹,², AKalpana Mujoo¹, Shashank Hambarde¹,³, Dharmendra Sharma¹, Abid R. Mattoo¹, Sharmistha Chakraborty¹,², Vijaya Charaka¹, Clayton R. Hunt¹,², Tej K. Pandita¹,²,

¹Department of Radiation Oncology, Houston Methodist Research Institute, Houston, TX, USA
²Department of Radiation Oncology, University of Texas Southwestern Medical School, Dallas, TX, USA
³Department of Microbiology and Molecular Genetics, McGovern Medical School, University of Texas Health Science Center at Houston, Houston, TX, USA

Published: April 24, 2026

This article has been corrected:It was identified that a portion of Figure 6B was improperly placed, resulting in an overlap between two images (Sptbn1−/− and Sptbn1+/+) for new DNA replication forks in MEF cells without Hydroxyurea treatment. The authors provided a revised Figure 6B, replacing the image for Sptbn1−/− cells without Hydroxyurea treatment with the correct data from the original experiments. This correction does not alter the article’s conclusions.

Original article: Oncotarget. 2016; 7:33557-33570. DOI: https://doi.org/10.18632/oncotarget.9677.

Figure 6: Sptbn1−/− MEFs exhibit defective stalled DNA replication fork resolution and new origins of replication. (A, B) Initiation of new DNA replication forks and reinitiation of stalled DNA replication forks in Sptbn1−/− and Sptbn1+/+ MEFs. cells were pre-labeled with 5-iododeoxyuridine (IdU), treated with hydroxyurea (HU) for the indicated intervals, and then rinsed to remove HU followed by post labeling with 5-chlorodeoxyuridine (CldU) (upper panel) as described previously [66]. The cells were fixed and immunostained with IdU (green) and CldU (red) antibodies. (A) Three major types of labeled DNA tracts for analysis are shown. (B) Sptbn1+/+ and Sptbn1−/− MEFs with and without treatment of hydroxyurea. (C) Percentages of stalled DNA replication forks (IdU only signals) in Sptbn1−/− and Sptbn1+/+ cells after HU treatment. (D) New origins (CldU signals) in Sptbn1−/− and Sptbn1+/+ cells. (E) Percentage of forks with fork speed in Sptbn1−/− and Sptbn1+/+ MEFs. Means ± standard deviations of 3 independent experiments are shown in. *p < 0.05; **p < 0.01, Student t-test.