Oncotarget

Research Papers:

miR-191 promotes tumorigenesis of human colorectal cancer through targeting C/EBPβ

Xiao-Fei Zhang _, Ke-ke Li, Lu Gao, Shang-Ze Li, Ke Chen, Jun-Bin Zhang, Di Wang, Rong-Fu Tu, Jin-Xiang Zhang, Kai-Xiong Tao, Guobin Wang and Xiao-Dong Zhang

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Oncotarget. 2015; 6:4144-4158. https://doi.org/10.18632/oncotarget.2864

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Abstract

Xiao-Fei Zhang1, Ke-ke Li1, Lu Gao3, Shang-Ze Li1, Ke Chen4, Jun-Bin Zhang5, Di Wang6, Rong-Fu Tu1, Jin-Xiang Zhang2, Kai-Xiong Tao2, Guobin Wang2,*, Xiao-Dong Zhang1,*

1College of Life Sciences, Wuhan University, Wuhan 430072, PR China

2Department of General Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430000, PR China

3Institute of Cardiovascular Disease, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430000, PR China

4Institute of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430000, PR China

5Department of Emergency, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430000, PR China

6Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430000, PR China

*These authors have contributed equally to this work

Correspondence to:

Xiao-Dong Zhang, e-mail: zhangxd@whu.edu.cn

Keywords: microRNA-191, colorectal cancer, apoptosis, C/EBPβ, tumorigenesis

Received: August 28, 2014     Accepted: December 07, 2014     Published: December 27, 2014

ABSTRACT

MicroRNA-191 (miR-191), a small non-coding RNA, is involved in disease development and cancer diagnosis and prognosis. However, how miR-191 functions in colorectal cancer remains largely unclear. In this study, we show that miR-191 is highly expressed in colon tumor tissues, and that inhibition of miR-191 leads to decreased cell growth, proliferation and tumorigenicity in a xenograft model. Overexpression of miR-191 in colorectal cancer cell lines alters cell cycle progression and cell resistance to 5-Fu induced cell apoptosis. Mechanistic studies demonstrated that miR-191 directly binds to the 3’UTR of the C/EBPβ mRNA and mediates a decrease in the mRNA and protein expression of C/EBPβ. We further showed that C/EBPβ induces growth arrest in a colorectal cancer cell line and that its expression is negatively correlated with the miR-191 level in patient samples. Our findings suggest that miR-191 may be a potential gene therapy target for the treatment of colorectal cancer.


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