Oncotarget

Research Papers:

Deciphering miR-520c-3p as a probable target for immunometabolism in non-small cell lung cancer using systems biology approach

Pooja Gulhane, Prajakta Nimsarkar, Komal Kharat and Shailza Singh

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Abstract

Pooja Gulhane1, Prajakta Nimsarkar1, Komal Kharat1 and Shailza Singh1

1 National Centre for Cell Science, NCCS Complex, Ganeshkhind, SP Pune University Campus, Pune 411007, India

Correspondence to:

Shailza Singh, email: singhs@nccs.res.in

Keywords: non-small cell lung cancer; miRNAs; miR-520c-3p; PI3K/AKT signaling pathway; therapeutic targeting

Received: December 13, 2021     Accepted: May 03, 2022     Published: May 24, 2022

Copyright: © 2022 Gulhane et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

ABSTRACT

Background: Non-small cell lung cancer (NSCLC) is considered to have more than 80% of all lung cancer cases, making it the leading cause of cancer-related deaths globally. MicroRNA (miRNA) deregulation has been seen often in NSCLC and has been linked to the disease’s genesis, progression, and metastasis via affecting their target genes.

Materials and Methods: Our study focused on the functionality of down-regulated miRNAs in NSCLC. For this study, we used 91 miRNAs reported to be down-regulated in NSCLC. The targets of these miRNAs were chosen from miRNA databases with functionality in NSCLC, including miRBase, miRDB, miRTV, and others. Inter-regulatory miRNA-NSCLC networks were generated. Simulated annealing was used to improve the network’s resilience and understandability. GSEA was used to examine 24607 genes reported experimentally in order to gain physiologically relevant information about the target miR-520c-3p.

Results: The study revealed functional prominence on miR-520c-3p, down-regulated during NSCLC. The involvement of miR-520c-3p in the PI3K/AKT/mTOR signaling pathway was recognized.

Conclusions: The therapeutic usage by designing a synthetic circuit of miR-520c-3p was explored, which may help in suppressing tumors in NSCLC. Our study holds promise for the successful deployment of currently proposed miRNA-based therapies for malignant disorders, which are still in the early pre-clinical stages of development.


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