Accumulation of FOXP3+T-cells in the tumor microenvironment is associated with an epithelial-mesenchymal-transition-type tumor budding phenotype and is an independent prognostic factor in surgically resected pancreatic ductal adenocarcinoma
PDF | HTML | Supplementary Files | How to cite
Metrics: PDF 2759 views | HTML 2252 views | ?
Martin Wartenberg1,2, Inti Zlobec2, Aurel Perren1,2, Viktor Hendrik Koelzer1,2, Beat Gloor3, Alessandro Lugli1,2, Eva Karamitopoulou1,2
1Clinical Pathology Division, University of Bern, Bern, CH-3010, Switzerland
2Translational Research Unit, Institute of Pathology, University of Bern, Bern, CH-3010, Switzerland
3Department of Visceral Surgery, Insel University Hospital, Bern, CH-3010, Switzerland
Eva Karamitopoulou, e-mail: [email protected]
Keywords: FOXP3, CD8, Tumor-associated macrophages, pancreatic cancer, prognosis, immune cell infiltration, epithelial mesenchymal transition, tumor budding, tumor microenvironment
Received: August 28, 2014 Accepted: November 18, 2014 Published: February 10, 2015
Here we explore the role of the interplay between host immune response and epithelial-mesenchymal-transition (EMT)-Type tumor-budding on the outcome of pancreatic adenocarcinoma (PDAC).
CD4+, CD8+, and FOXP3+T-cells as well as iNOS+ (M1) and CD163+- macrophages (M2) were assessed on multipunch tissue-microarrays containing 120 well-characterized PDACs, precursor lesions (PanINs) and corresponding normal tissue. Counts were normalized for the percentage of tumor/spot and associated with the clinico-pathological features, including peritumoral (PTB) and intratumoral (ITB) EMT-Type tumor-budding and outcome.
Increased FOXP3+T-cell-counts and CD163-macrophages and decreased CD8+T-cell-counts were observed in PDACs compared with normal tissues and PanINs (p < 0.0001). Increased peritumoral FOXP3+T-cell-counts correlated significantly with venous invasion, distant metastasis, R1-status, high-grade ITB, PTB and independently with reduced survival. Increased intratumoral FOXP3+T-cells correlated with lymphatic invasion, N1-stage, PTB and marginally with adverse outcome. High peritumoral CD163-counts correlated with venous invasion, PTB and ITB. High intratumoral CD163-counts correlated with higher T-stage and PTB.
PDAC-microenvironment displays a tumor-favoring immune-cell composition especially in the immediate environment of the tumor-buds that promotes further growth and indicates a close interaction of the immune response with the EMT-process. Increased peritumoral FOXP3+T-cell density is identified as an independent adverse prognostic factor in PDAC. Patients with phenotypically aggressive PDACs may profit from targeted immunotherapy against FOXP3.
All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.