By downregulating TIAM1 expression, microRNA-329 suppresses gastric cancer invasion and growth
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Zheng Li1, Xin Yu1, Yang Wang2, Jianxiong Shen1, William Ka Kei Wu3, Jinqian Liang1, Fan Feng1
1Department of Orthopaedic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
2Department of Abdominal Surgery, Cancer Institute and Cancer Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China
3Department of Anaesthesia and Intensive Care, The Chinese University of Hong Kong, Hong Kong, China
Jianxiong Shen, e-mail: firstname.lastname@example.org
Keywords: Gastric cancer, MicroRNAs, miR-329, TIAM1
Received: November 09, 2014 Accepted: November 16, 2014 Published: February 11, 2015
Gastric cancer (GC) is one of the most common malignant tumors worldwide. Emerging evidence has shown that abnormal microRNAs (miRNAs) expression is involved in tumorigenesis. MiR-329 was previously reported to act as a tumor suppressor or oncogene in some types of cancer. However, its function in gastric cancer (GC) is unclear. Here, we found that miR-329 was down-regulated in GC compared with adjacent controls. Enforced expression of miR-329 inhibited proliferation, migration and invasion of gastric cancer cells in vitro. We identified T lymphoma invasion and metastasis 1 (TIAM1) gene as potential target of miR-329. MiR-329 levels inversely correlated with TIAM1 expression in GC. Importantly, TIAM1 rescued the miR-329-mediated inhibition of cell invasion and proliferation. Finally, reintroduction of miR-329 significantly inhibited tumor formation of GC in the xenograft mice. Our findings suggest that miR-329 is a tumor suppressor and potential therapeutic target of GC.
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